上转换纳米颗粒荧光寿命测量数据采集与处理

Data acquisition and processing for UCNPs fluorescence lifetime measurement

为了测试分析上转换纳米材料(up-conversion nanoparticles, UCNPs)的荧光寿命,设计了一种针对UCNPs进行荧光寿命信号数据高速采集并测量输出的系统。该系统通过MATLAB编写虚拟原始荧光信号并完成滤波和数据拟合,将数据程序脚本嵌入LabVIEW中进行计算和处理,在上位机显示荧光信号波形、拟合数据波形、荧光个数、荧光寿命以及寿命极值等。研究结果表明:该系统软件仿真和数值计算模拟取得了基本一致的结果,在背景噪声一定的情况下,两者误差在3%以内,具有良好的稳定性和准确性。本研究工作对UCNPs荧光寿命测量系统的研发有一定指导价值。

In order to test and analyze the fluorescence life of up-conversion nanoparticles (UCNPs), a system was designed for high-speed acquisition and measurement of fluorescence signal data of UCNPs. The system programmed the virtual original fluorescent signals through MATLAB, completed the filtering and data fitting, embeded the data program script into LabVIEW for calculating and processing, and displayed the fluorescent signal waveform, fitting data waveform, fluorescence number, fluorescence life and extreme of the life on the host computer. The research results show that the system software simulation and numerical calculation simulation have achieved basically consistent results, the error between the two is less than 3%, with good stability and accuracy. This research work has great guiding value for the development of UCNPs fluorescence lifetime measurement system.