辣椒酸性蔗糖转化酶基因家族鉴定及表达

Genome Identification and Expression of Acid Invertase Gene in Pepper

为了探究辣椒酸性蔗糖转化酶基因的多样性,本研究根据已公布的辣椒基因组数据信息,利用生物信息学方法对辣椒酸性蔗糖转化酶(Acid invertase)基因家族成员进行鉴定,对其染色体定位、系统发育树关系、表达模式进行分析。结果表明:辣椒含有9个酸性蔗糖转化酶基因成员(CaVIN01~CaVIN02,CaCWIN01~CaCWIN07),2个属于液泡蔗糖转化酶,7个属于细胞壁蔗糖转化酶。其编码氨基酸长度在513~656 aa之间,分子量大小位于58.19~73.31 kD之间,等电点变化在5.49~8.97区间;染色体定位发现,其中8个基因分布于四条染色体上,CaCWIN06的染色体定位是不确定的;系统发育树的构建来自于4个物种:辣椒、番茄、拟南芥和水稻,系统发育树显示酸性蔗糖转化酶分为两类:液泡蔗糖转化酶(vacuolar invertase, VIN)和细胞壁蔗糖转化酶(cell wall invertase, CWIN),其中细胞壁蔗糖转化酶又分为四个亚支(Ⅰ~Ⅳ);基于RNA-seq分析发现,CaVIN02和CaCWIN03在花和果实发育中呈现高表达,其它基因表达量较低;叶片中CaVIN02和CaCWIN07具有较高表达量,其它基因表达量低;进一步研究发现,在激素胁迫下,CaVIN01、CaVIN02、CaCWIN01、CaCWIN02和CaCWIN07受到激素的诱导,而其它基因几乎无变化;高低温胁迫下CaVIN02和CaCWIN03具有明显的表达量,这两个基因受温度诱导最为明显,其它基因无明显表达。这些结果为深入探讨辣椒蔗糖转化酶基因的功能提供数据基础。

In order to explore the diversity of pepper acid invertase genes, based on the published pepper genome data, the members of pepper acid invertase gene family, their chromosome location, phylogenetic tree relationship and expression patterns were identified by bioinformatics methods. The results showed that pepper contains 9 acid invertase gene members (CaVIN01~CaVIN02, CaCWIN01~CaCWIN07), 2 belong to vacuolar invertase and 6 belong to cell wall invertase. The length of the encoded amino acid was between 513~656 aa, the molecular weight was between 58.19~73.31 kD, and the isoelectric point was between 5.49~8.97. It was found that eight genes were distributed on four chromosomes, and CaCWIN06's chromosome location was uncertain. Phylogenetic tree built from four species: the hot pepper, tomato, Arabidopsis and rice, the phylogenetic tree divides acid sucrose invertase into two categories: vacuole of sucrose invertase (vacuolar invertase, VIN) and the Cell walls of sucrose invertase (cell wall invertase, CWIN), and the Cell walls of sucrose invertase is divided into four branch (Ⅰ~Ⅳ), which is consistent with the classification of tomato;Based on RNA-seq analysis, it was found that CaVIN02 and CaCWIN03 were highly expressed in flower and fruit development, while the expression levels of other genes were low. The expression levels of CaVIN02 and CaCWIN07 in leaves were medium and high, while the expression levels of other genes were low. Further study found that under hormone stress, CaVIN01, CaVIN02, CaCWIN01, CaCWIN02 and CaCWIN07 were induced by hormones, while other genes were almost unchanged. CaVIN02 and CaCWIN03 had significant expression under high and low temperature stress, and these two genes were most obviously induced by temperature, while other genes were not significantly expressed. These results lay a foundation for further study on the function of invertase gene in pepper.