植物赤霉素氧化酶GA20ox基因的生物信息学分析

Bioinformatics Analysis of Gibberellin Oxidase GA20ox Gene in Plants

在植物发育和生理过程中,GA-20氧化酶(GA-20 oxidase, GA20ox)起着非常重要的调控作用。运用生物信息学的方法,对27种不同植物的GA20ox基因的氨基酸序列的基本理化参数进行预测和分析,并构建其系统进化树。结果表明,植物GA20ox的蛋白质序列长度在345~426 aa之间,分子量在39.23~49.15 kD之间,等电点在5.09~7.65之间,大多数植物的GA20ox蛋白呈酸性、蛋白较为稳定、表现为亲水性、定位于细胞核中、具有多个功能位点(包括磷酸化位点和糖基化位点);27种不同植物的含有5个保守基序,其中Motif 1和Motif 2为2OG-FeⅡ_Oxy域,Motif 3为DIOX_N域;基于氨基酸序列的系统聚类结果与物种间的亲缘关系远近相一致,具有明显种属特征;GA20ox蛋白二级结构以无规则卷曲和α-螺旋为主要元件。该研究结果对深入解析植物GA20的生物学功能及GA调控机理具有重要意义,为以后植物定向改良育种实践提供理论参考。

Gene gibberellin 20-oxidase(GA20 ox) plays a key role in the growth development and physiological processes of plants. In this study, the basic physical and chemical parameters of amino acid sequence of GA20 gene in 27 kinds of plants were predicted and analyzed by bioinformatics methods, to construct its systematic evolution tree. The results showed that protein sequence length of plant GA20 ox was ranged from 345~426 aa,molecular weight was ranged from 39.23~49.15 kD, and theoretical isoelectric point(pI) was ranged from 5.09~7.65. Most GA20 ox proteins were acidic and more stable, it shown as hydrophilic, and were located in the nucleus,with multiple functional sites(including phosphorylation sites and glycosylation sites). GA20 ox proteins from 27 kinds of plants contained 5 conserved motifs, in which Motif 1 and Motif 2 were 2OG-FeⅡ_Oxy domain, Motif 3 was DIOX_N domain. The results of systematic clustering based on amino acid sequence were consistent with the genetic relationship between species, and with obvious species and genus characteristics. The secondary structure showed that the main structural elements of GA20 ox protein were random coil and α-helix. This study are helpful for resolving the structures and biological functions of plant GA20 ox and GA regulating mechanism, and providing an important reference for the directional improvement of plant traits.