基于trnH-psbA和matK序列的油茶种子DNA条形码鉴定

DNA Barcoding Identification of Camellia spp. Seed Based on trnH-psbA and matK Sequences

本研究利用叶绿体基因组trnH-psbA和matK序列对不同物种的34份油茶种子进行DNA条形码鉴定,以分析这两个序列在鉴别越南油茶(Camellia vietnamensis)和普通油茶(Camellia oleifera)的效率。结果表明:34份供试材料的trnH-psbA序列全长395 bp、11个变异位点,系统发育树将普通油茶聚为一支,支持率为72%;matK序列全长837 bp、2个变异位点,系统发育树将越南油茶与高州油茶聚为一支,支持率为64%。可见,trnH-psbA序列和mat K序列结合,可对普通油茶和越南油茶与其他供试油茶物种进行有效鉴别。

In this paper, DNA barcoding identification of 34 Camellia oleifera Abel. seeds of different species was performed by using chloroplast genome trnH-psbA and matK sequences, so as to analyze the efficiency of the two sequences distinguishing Camellia vietnamensis and C. oleifera. The results showed that in 34 test materials the total length of trnH-psbA sequence was 395 bp with 11 mutation loci. Phylogenetic trees cluster C. oleifera into one branch, with 72% bootstrap support. The total length of matK sequence is 837 bp with 2 mutation loci. Phylogenetic trees cluster Camellia vietnamensis and Camellia gauchowensis into one branch, with 64% bootstrap support. It can be seen that the combination of trnH-psbA sequence and matK sequence can effectively identify Camellia vietnamensis and C. oleifera.