不同产地滇黄精丛枝菌根真菌、深色有隔内生真菌定殖调查及与主要功效成分含量相关性分析

Colonization investigation of arbuscular mycorrhizal fungi(AMF) and dark septate endophytes(DSE) in roots of Polygonatum kingianum and their correlations with content of main functional components in rhizomes

目的调查不同产地滇黄精根系中丛枝菌根真菌(AMF)和深色有隔内生真菌(DSE)定殖情况,并探讨其与主要功效成分之间的相关性。方法以产自于云南省5个样地的滇黄精为研究对象,采用碱解离-酸性品红染色法,调查AMF和DSE在滇黄精须根部位的定殖情况,并对典型形态结构进行拍照分析;采用硫酸苯酚法、香草醛-冰醋酸-高氯酸比色法、分光光度法和醇溶热浸法测定滇黄精根状茎中多糖、薯蓣皂苷元、总黄酮和浸出物含量,并通过皮尔森相关系数法分析AMF及其典型结构定殖率、DSE及其典型结构定殖率与4种功效成分含量之间的相关性。结果在5个样地中,AMF和DSE在滇黄精须根中的平均定殖率范围分别为26.25%~57.54%、31.67%~45.19%,其中样地红河州蒙自县(HHMZ)AMF和DSE定殖率均高于其他样地。4种功效成分与AMF、DSE定殖率及典型结构定殖率之间均呈正相关,其中多糖、薯蓣皂苷元和总黄酮含量与AMF、DSE定殖率相关性相对较高,AMF/DSE相关系数分别为0.838/0.887、0.819/0.703、0.785/0.855,且多糖含量与AMF菌丝定殖率、DSE定殖率呈显著相关。此外,AMF及典型结构定殖率与DSE及典型结构定殖率之间、4种功效成分含量两两之间均存有较高的相关性。结论AMF和DSE在滇黄精须根中有着较高的定殖率,且与主要功效成分含量之间呈正相关。本研究为实施滇黄精生态种植、采用生物手段增产增收提供了数据支持和实验依据。

Objective To investigate the colonization of arbuscular mycorrhizal fungi(AMF)and dark septate endophytes(DSE)in the roots of Polygonatum kingianum planted at different areas,and explore the correlations between the colonization rate and the main functional components.Methods Fresh roots of P.kingianum from five plots in Yunnan Province were taken as research objects.The alkali dissociation method was used to investigate the colonization of AMF and DSE in the roots of P.kingianum.Then the morphological structure was photographed.The content of P.sibiricum polysaccharides,diosgenin,total flavonoids and extractum were separately determined by phonel-sulfate method,colorimetry method of vanillin-acetic acid-perchloric acid,spectrophotometry method with rutin standard and hot dipping method with alcohol.The correlations between the colonization rates of AMF or DSE and four main functional components were analyzed by Pearson correlation coefficient method.Results The average colonization rates of AMF and DSE in the five plots were 26.25%-57.54%and 31.67%-45.19%,respectively.The colonization rates of AMF and DSE from HHMZ was higher than the other four others.All of correlations among the colonization rates of AMF,DSE and their typical structure and the four main functional components in the rhizomes were positive correlation,in which the correlations between the content of polysaccharides,diosgenin or total flavonoids and the colonization rates of AMF or DSE were higher,and their correlation coefficient were respectively 0.838/0.887,0.819/0.703,and 0.785/0.855(AMF/DSE).Furthermore,the correlations between the content of polysaccharides and the colonization rates of AMF hypha or DSE were significant.In addition,there were high correlations among the colonization rates of AMF,DSE and their typical structures,as well as the content in pairs of four functional components.Conclusion There were higher colonization rates of AMF and DSE in the fibrous roots of P.kingianum.The correlations between the colonization rates of AMF or DSE and each main functional component were positive.This study provides data support and experimental basis for the implementation of ecological planting of P.kingianum and the use of biological means to increase production and income.