番木瓜实生苗两性株的鉴定及其组培繁殖体系的建立

Identification and Establishment of Tissue Propagation System of Hermaphroditic Strains of Papaya Seedling

本研究以多重PCR技术鉴定的番木瓜实生苗两性株茎尖作为外植体,建立和优化了一套组培苗繁殖体系,解决了成龄侧芽来源的无根苗催根难和移栽成活率低的问题。以'蔬罗''蜜红'番木瓜品种的实生苗,通过多重PCR鉴定出两性株,将其茎尖培养于MS+BA0.5mg/L+NAA0.1mg/L+蔗糖30g/L+琼脂6g/L(pH5.8)上,在28℃、2000lx条件培养30d后,继代于MS+BA0.5mg/L+KT0.25mg/L+NAA0.1mg/L+蔗糖30g/L+琼脂6g/L(pH5.8)上,在28℃、2000lx条件下培养30d形成较为强壮的无根苗,接种于1/2MS+IBA0.75mg/L+NAA0.05mg/L+KT0.01mg/L+蔗糖30g/L+琼脂6g/L(pH5.8)上进行催根,在28℃、1500lx条件下培养20d后,用不同浓度营养生根水和不同处理时间对'蔬罗''蜜红'无根苗进行移栽试验,以确定最佳催根率和移栽成活率的浓度和时间。研究结果表明:多重PCR技术鉴定性别的准确率达98%以上,'蔬罗'在50mg/L营养生根水和8h处理条件下获得催根率为81.1%、移栽成活率为91.1%。而'蜜红'品种则催根率为60%、移栽成活率为86.7%。因此利用这一技术获得的结果相比成龄侧芽优势明显,可用于番木瓜种苗的商业化生产。

In this study,the apical stem of the hermaphroditic strain of papaya seedlings identified by multiple PCR technique was used as the explants to establish and optimize a set of propagation system of papaya seedlings in tissue culture. The problem of rootless seedlings and low survival rate of transplanting were solved. In the experiment,the hermaphrodite plants were identified by multiple PCR and the stem tips were cultured on MS supplement with BA 0.5 mg/L,NAA 0.1 mg/L,sucrose 30 g/L,agar 6g/L (pH 5.8),and cultured at 28 ℃for 30 days. The rootless seedlings were formed and subdivided into small clusters and planted on the strong seedling medium MS supplement with BA 0.5 mg/L, KT 0.25 mg/L,NAA 0.1 mg/L,sucrose 30 g/L,agar 6g/L (pH 5.8),cultured at 28 ℃and 2000 lx for 30 days. The rootless seedlings were inoculated on the 1/2MS supplement with IBA 0.75 mg/L,NAA 0.05 mg/L,KT 0.01 mg/L,sucrose 30 g/L,agar 6 g/L (pH 5.8) medium. After being cultured at 28 ℃and 1500 lx for 20 days. In order to obtain the best rooting rate and survival rate of transplanting,the rootless seedlings were tested with different concentrations of nutrient rooting water and different treatment time. The results showed that the accuracy of multiple PCR technique for sex identification was over 98%. Under the conditions of 50 mg/L nutrient rooting water and 8 h treatment,the rooting rate of 'Solo' was 81.1%,and the survival rate of transplanting was 91.1%. The rooting rate of 'Mihong' was 60% and the survival rate of transplanting was 86.7%. The results obtained by the technique are superior to those of mature lateral buds,and could be used in the commercial production of papaya seedlings.