摘要
本试验利用Blue Light Broth "Nissui"和MRS培养基对门源县饲喂羊的青贮饲料中的菌种进行了分离分纯和16S rRNA序列通用引物PCR扩增和测序鉴定,分离分纯得到12株菌,其中8株为地衣芽孢杆菌,1株为单纯芽孢杆菌,2株为干酪乳杆菌,1株为副乳杆菌。
In this experiment, Blue Light Broth "Nissui" and MRS medium were used to isolate and identify the strains of silage fodder fed to sheep in Qilian County, Qinghai Province, and PCR amplification and sequencing identification of 16 S rRNA sequences. The results showed that 12 strains of bacteria were isolated and separated. Among them, 8 strains were Bacillus licheniformis strain, 1 strain was Bacillus simplex strain, 2 strains were Lactobacillus casei strain, and 1 strain was Lactobacillus paracasei strain.
作者
胡勇
简莹娜
刘文先
李世双
李秀萍
王戈平
马利青
HU Yong(Accdemy of Animal Sciences and Veteonary Medicine of Qinghai University, Xining 810016)
出处
《青海畜牧兽医杂志》
2019年第4期1-5,共5页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
国家农业产业技术体系经费资助(CARS-39-06B)
关键词
门源县
青贮燕麦草
菌种
分离
鉴定
Menyuan County
Silage oats
Bacterial strain
Separation
Identify
