小伞山羊草染色体的FISH核型分析

FISH Karyotype Analysis of Chromosomes in Aegilops umbellulata

采用荧光原位杂交(Fluorescence in Situ Hybridization,FISH)技术分析小伞山羊草染色体的核型特点。结果表明,小伞山羊草共包含7对染色体,其中1 U和5 U染色体各含有1对随体。分别以Oligo-pSc 119.2-1(绿色)与(GAA)7(红色)重复序列为探针对小伞山羊草根尖细胞染色体进行FISH分析,发现Oligo-pSc-119.2-1信号主要分布在染色体的端部及近端部,不同染色体之间的信号强度有所差别。(GAA)7信号主要集中于染色体着丝点附近,不但比Oligo-pSc-119.2-1信号的亮度高,分布丰富,而且在染色体上的分布可与Oligo-pSc-119.2-1信号互补。因此,同时采用Oligo-pSc-119.2-1与(GAA)72种探针能准确地辨别小伞山羊草的每对染色体,建立了小伞山羊草的FISH核型。

FISH(Fluorescence in situ hybridization)technique was applied to analyze chromosome patterns of Aegilops umbellulata.The results showed that Aegilops umbellulata contained a total of 7 pairs of chromosomes,of which 1 U and 5 U chromosomes had a pair of satellites,respectively.Using the Oligo-pSc 119.2-1(green)and(GAA)7(red)repetitive sequence as probes,chromosome FISH analysis of the root tip cells in Aegilops umbellulata showed that the Oligo-pSc-119.2-1 signals were mainly distributed in the telomeres and proximal telomeres of chromosomes,and the signal intensity varied among different chromosomes.The(GAA)7 signals were generally concentrated near the centromeres of the chromosomes,which were not only more luminous and abundant than those of Oligo-pSc-119.2-1 signals,but also complementary distribution to those of Oligo-pSc-119.2-1 signals on chromosomes.Therefore,both Oligo-pSc 119.2-1 and(GAA)7 probes were used to accurately identify each pair of chromosomes in Aegilops umbellulata,and its FISH karyotype was established.