响应面法优化橘皮中黄色素的超声辅助提取工艺

Optimization of Ultrasonic-Assisted Extraction Process of Yellow Pigment in Orange Peel by Response Surface Methodology

本论文采用超声辅助提取工艺,在单因素实验的基础上,探究了乙醇浓度、超声时间、温度、料液比对提取液吸光度的影响。利用Box-Benhnken实验设计研究优化了橘皮中黄色素的提取的工艺条件,并研究了温度和常见金属离子对该黄色素稳定性的影响。采用响应面法对超声辅助提取橘皮中黄色素工艺进行优化的结果为:乙醇浓度70%、料液比1∶5、超声时间45min、温度70℃,最佳吸光度为0.773。研究发现温度对黄色素基本无影响,Fe3+离子对橘皮黄色素有破坏作用,Na+、K+、Cu2+离子则无明显的影响。

Based on the single factor experiment, this dissertation adopted the ultrasonic-assisted extraction technology to study the effect of ethanol concentration, ultrasonic time, temperature and ratio of material to liquid on the absorbance of the solution of extraction. The Box-Benhnken experimental design was used to optimize the extraction technology of yellow pigment in orange peel, the research also studies the effect of temperature and common metal ions on the stability of the yellow pigment from orange peel. The optimized result by using optimization of ultrasonic extraction technology for yellow pigment in orange peel by response surface methodology: ethanol concentration 70 %, liquid ratio 1∶5, ultrasonic time 45 min, temperature 70 ℃, and the optimum absorbance was 0.773. The temperature has no effect on that yellow pigment. Fe3+ ion has a destructive effect on the yellow pigment extracted from orange peel, but Na+, K+ and Cu2+ ions have no obvious influence on that yellow pigment.