川芎嗪防护严重烧伤大鼠急性肺损伤及对NF-κB/NLRP3信号通路的影响

Protective Effect of Ligustrazine on Acute Lung Injury in Severely Burned Rats and Its Effect on NF-κB/NLRP3 Signaling Pathway

目的探讨川芎嗪对严重烧伤大鼠急性肺损伤(ALI)的防护作用及核因子κB(NF-κB)/NLRP3信号通路的影响。方法将60只SD大鼠随机分成对照组、模型组、川芎嗪组,其中模型组、川芎嗪组构建严重烧伤大鼠ALI模型,并于造模成功后给予相应药物腹腔注射,对照组和模型组分别给予37℃温水和等体积生理盐水。结果与对照组比较,模型组、川芎嗪组大鼠湿肺/体质量比值、肺损伤评分明显升高,动脉氧分压(PaO2)水平明显降低(P<0.05);与模型组比较,川芎嗪组大鼠湿肺/体质量比值、肺损伤评分明显降低,PaO2水平明显升高(P<0.05)。与对照组比较,模型组、川芎嗪组肺NF-κBmRNA及NLRP3mRNA、蛋白表达水平均明显升高(P<0.05);与模型组比较,川芎嗪组大鼠肺NF-κBmRNA及NLRP3mRNA、蛋白表达水平均明显降低(P<0.05)。与对照组比较,模型组、川芎嗪组大鼠IL-2,IL-6,TNF-α蛋白表达水平均明显升高(P<0.05);与模型组比较,川芎嗪组大鼠IL-2,IL-6,TNF-α蛋白表达水平均明显降低(P<0.05)。结论川芎嗪对严重烧伤大鼠ALI具有防护作用,其机制可能与川芎嗪能抑制炎症信号通路NF-κBmRNA和NLRP3mRNA、蛋白表达水平,从而抑制炎性因子IL-2,IL-6,TNF-α的表达有关。

Objective To investigate the protective effect of ligustrazine on acute lung injury in severely burned rats and its effect on NF-κB/NLRP3 signaling pathway. Methods Totally 60 SD rats were randomly divided into the control group,model group and ligustrazine group. The rats in the model group and ligustrazine group were used to establish acute lung injury models of severely burned rats,and the corresponding drugs were injected intraperitoneally at the beginning of the establishment of the models. The rats in the control group were given warm water( 37 ℃) and those in the model group were given the same amount of normal saline. Results Compared with those in the control group,the lung/body weight and the scores of lung injury increased significantly,and the level of the partial pressure of arterial oxygen( PaO2) decreased significantly in the ligustrazine group and model group( P < 0. 05). Compared with those in the model group,the lung/body weight and the scores of lung injury decreased significantly,and the level of PaO2 increased significantly in the ligustrazine group( P < 0. 05). Compared with those in the control group,the expression levels of NF-κB m RNA,NLRP3 m RNA and protein in lung increased significantly in the model group and ligustrazine group( P < 0. 05). Compared with those in the model group,the expression levels of NF-κB m RNA,NLRP3 m RNA and protein in lung decreased significantly in the ligustrazine group( P < 0. 05). Compared with those in the control group,the expression levels of interleukin( IL-2),IL-6 and tumor necrosis factor-α( TNF-α) protein increased significantly in the model group and ligustrazine group( P < 0. 05). Compared with those in the model group,the expression levels of IL-2,IL-6 and TNF-α protein decreased significantly in the ligustrazine group( P <0. 05). Conclusion Ligustrazine has protective effect on acute lung injury in severely burned rats. Its mechanism may be related to the inhibition of expression levels of NF-κB m RNA and NLRP3 m RNA and protein in the inflammatory signaling pathway,as well as inhibition of expression levels of inflammatory factors such as IL-2,IL-6 and TNF-α.