阿奇霉素对牙龈卟啉单胞菌脂多糖刺激牙龈成纤维细胞产生前列腺素E2影响的研究

Effect of azithromycin on the production of prostaglandin E2 by gingival fibroblasts stimulated by Porphyromonas gingivalis lipopolysaccharide

目的探究不同浓度阿奇霉素(Azithromycin,AZM)对牙龈卟啉单胞菌脂多糖(Porphyromonas gingivalis lipopolysaccharide,PgLPS)刺激人牙龈成纤维细胞(human gingival fibroblasts,HGFs)产生前列腺素E2(Prostaglandin E2,PGE2)的影响。方法 WST-1法检测0.1~10ug/mlAZM及10ug/mlPgLPS对HGFs增殖活性影响,ELISA法检测上述浓度AZM和PgLPS对细胞产生PGE2的影响。结果各实验组与对照组相比在细胞增殖活性吸光度值方面差异无统计学意义。10 ug/mlPgLPS组可以显著促进HGFs产生PGE2(512.60±10.26 pg/ml),差异有统计学意义(P<0.05),在1 ug/mlAZM作用下细胞上清液中检测PGE2含量最低(153.81±8.89 pg/ml),差异有统计学意义(P<0.05)。结论 PgLPS刺激HGFs产生炎症因子PGE2,促进牙槽骨吸收;1 ug/mlAZM对PgLPS刺激HGFs产生PGE2的抑制作用最强。

Objective To investigate the effects of different concentrations of Azithromycin (AZM) on Porphyromonas gingivalis lipopolysaccharide LPS (PgLPS) to stimulate human gingival fibroblasts (HGFs) to produce prostaglandin E2 (PGE2).Methods WST-1 method detected the effect of 0.1-10ug/mlAZM and 10ug/ mlPgLPS on HGFs proliferation activity,the effect of the above concentrations of AZM and PgLPS on the production of PGE2 was detected by ELISA.Results Compared with the control group there was no significant difference in the absorbance value of cell proliferation activity.The 10ug/ml PgLPS group could significantly promote the production of PGE2 by HGFs (512.60±10.26pg/ml),the difference was statistically significant (P<0.05),and the cells were treated with 1ug/ml AZM,The PGE2 content in the supernatant was the lowest (153.81±8.89pg/ml),the difference was statistically significant (P<0.05).Conclusion PgLPS stimulated HGFs to produce inflammatory factor PGE2 and promote alveolar bone resorption;1ug/ml AZM stimulated PgLPS HGFs produces the strongest inhibitory effect of PGE2.