构树染色体制片效果影响因素与核型分析

Influencing Factors of Tabletting Chromosome of Broussonetia papyrifea and Karyotype Analysis

于2017年10月采集广东省广州市的构树Broussonetia papyrifea种子5 000粒,2018年2月进行种子萌发,通过常规压片方法进行核型分析。结果表明,在2018年3月2日至5月9日的10:00-10:30,发育7 d的材料有丝分裂指数最高,相比于化学处理方法,用冰水混合物预处理12h时染色体形态更清晰易辨;解离10min时根尖细胞染色体分散较好。构树染色体数目为26条,为二倍体,核型公式为2 n=2 x=26 m,核型属于2A型。

In October of 2017, 5000 seeds were collected from Broussonetia papyrifea in Guangzhou, Guangdong province, and in February of 2018, germination breaking was carried out. Caryotype analysis was implemented by pellet method. The result showed that the highest mitotic index was obtained from 7-day radicals at 10:00 and 10:30 during March 2 to May 9 of 2018. The morphology of chromosome was clearer when the radicals were treated with ice water for 12 hours. The dispersion of chromosomes was the best when 10 minutes was dissociated. The chromosome numbers of B. papyrifera were 26, diploid, and the karyotype formula was 2 n=2 x=26 m, and the karyotype belonged to 2 A type.