醋膏多糖和多肽的组成分析

Analysis of polysaccharides and polypeptides in cereal vinegar sediment

对醋膏中多糖和多肽的含量及组成进行初步分析,并将其与醋液中乙醇沉淀分离的大分子物质进行对比.检测醋膏与醋液醇沉物中多糖的含量及多糖的硫酸基含量和单糖组成,多肽的含量及水解氨基酸组成.醋膏和醋液醇沉物中多肽和多糖相对含量总和分别为67.4 g/100 g和73.0 g/100 g.醋膏和醋液醇沉物粗多糖中均含有6种单糖,但氨基葡萄糖的相对含量(27.4%)明显高于醋液醇沉物(3.2%).醋膏和醋液醇沉物粗多糖中硫酸基含量分别为2.6%和0.3%.醋膏和醋液醇沉物的多肽均含有17种水解氨基酸,其中必需氨基酸分别占氨基酸总量的27.4%和19.5%,支链氨基酸占15.9%和7.7%.醋膏主要由多糖、多肽等物质组成,醋膏的多肽营养价值高于醋液醇沉物.该研究为了解醋膏成分提供了参考数据.

The contents and compositions of polysaccharides and polypeptides in cereal vinegar sediment and vinegar ethanol precipitate were analyzed. The polysaccharides content,the sulfate group content and monosaccharide composition of polysaccharides,and the polypeptides content and hydrolyzed amino acids compositions were determined. The relative contents of polypeptides and polysaccharides found to be 67.4 g/100 g and 73.0 g/100 g in the vinegar sediment and vinegar ethanol precipitate,respectively. There were six kinds of monosaccharides in crude polysaccharides of vinegar sediment and vinegar ethanol precipitate. The relative content of glucosamine (27.4%) in the polysaccharides from vinegar sediment was significantly higher than that from vinegar ethanol precipitate (3.2%). The sulfate group content of crude polysaccharides in vinegar sediment and vinegar ethanol precipitate were 2.6% and 0.3%,respectively. There were 17 kinds of hydrolyzed amino acids in vinegar sediment and vinegar ethanol precipitate. The vinegar sediment had 27.4% essential amino acids and 15.9% branched amino acids,while the vinegar ethanol precipitate had 19.5% essential amino acids and 7.7% branched amino acids. Therefore,the nutritional value of polypeptides in vinegar sediment is higher than that in vinegar ethanol precipitate. This study overall provides references for understanding the components of vinegar sediment.