外周血miR-197表达水平与子宫肌瘤患者病情及治疗效果的相关性研究

Correlation between the Expression Level of miR-197 in Peripheral Blood and the Condition and Therapeutic Effect of Patients with Uterine Fibroids

目的:探究外周血miR-197表达水平与子宫肌瘤患者病情及治疗效果的相关性。方法:将100例行子宫肌瘤剔除术的患者作为观察组,选取同期体检的100例健康女性作为对照组,采用RT-qPCR的方法检测两组术前、术后外周血miR-197表达水平,测量子宫体积大小;采用免疫组化染色方法测定观察组患者切除肿瘤组织中雌激素受体(ER)的表达水平,测量肿瘤体积大小;分析患者外周血miR-197水平与ER表达水平、肿瘤大小的相关性。结果:观察组外周血miR-197表达水平明显低于对照组,差异有统计学意义( P <0.05);观察组术后外周血miR-197表达水平明显高于术前,差异有统计学意义( P <0.05);患者外周血miR-197表达水平与ER表达水平、肿瘤体积大小均呈负相关( P <0.05)。结论:外周血miR-197表达水平在一定程度上可以反映子宫肌瘤患者的肿瘤大小、ER表达水平,且治疗后患者外周血miR-197水平明显升高,是患者病情及治疗的良好观察指标。

Objective: To investigate the correlation between the expression of miR-197 in peripheral blood and the condition and therapeutic effect of patients with uterine fibroids. Methods: 100 patients with uterine fibroids who underwent hysteromyoma were treated as observation group.100 healthy women from the same physical examination were selected as the control group.RT-qPCR was used to detect the peripheral blood miR-197 expression level of the control group and the observation group before and after operation;Measuring the size of uterus;Immunohistochemical staining method was used to determine the expression level of estrogen receptor (ER) in the tumor tissue of the observation group, and the tumor volume was measured;Correlation between expression levels of peripheral blood miR-197 level and ER and tumor size were analyzed. Results: The expression of miR-197 in the peripheral blood of the observation group was significantly lower than that of the control group,the difference was statistically significant ( P <0.05);the expression level of the peripheral blood miR-197 was significantly higher than that before surgery, the difference was statistically significant( P <0.05);the expression of miR-197 in peripheral blood was negatively correlated with ER expression,tumor volume ( P <0.05). Conclusion: The expression level of miR-197 in peripheral blood can reflect the tumor size and ER expression level in patients with uterine fibroids, and the level of miR-197 in peripheral blood of patients significantly increased after treatment.It is a good observation index for patients’condition and treatment.