不同免疫检查点基因在急性髓细胞白血病中的表达与临床意义

The expression and clinical significance of immune checkpoints in acute myeloid leukemia

目的分析不同免疫检查点分子在急性髓细胞白血病(AML)中的临床意义,寻找潜在有效的免疫治疗靶点。方法使用GEO数据库下载肿瘤细胞系表达芯片数据(GSE57083)及AML表达芯片数据(GSE37642),然后使用R studio、Graph Pad软件分析各基因在肿瘤细胞中的表达及与患者生存时间的关系。结果 16种AML细胞系中11种免疫检查点相关基因的表达从高至低依次为LGALS9、PVRL2、PVR、PDCD1、TIM3、CTLA4、PDL1、GITR、LAG3、PDL2、GITRL,其中LGALS9、PVRL2、GITR在不同细胞间表达变异度较大;AML组织中各基因表达从高至低依次为LGALS9、PVRL2、TIM3、PDCD1、CTLA4、LAG3、GITR、PDL1、PVR、GITRL、PDL2;生存分析结果显示PVR、LGALS9基因的高表达与AML不良预后相关,PDCD1、PDL1、PDL2、CTLA4、LAG3、TIM3、PVRL2、GITR、GITRL等表达量高低与患者生存时间无相关性(P> 0.05)。结论 PVR在AML肿瘤细胞中高表达,LGALS9在AML肿瘤细胞与组织中均高表达,PVR与LGALS9的高表达与AML患者不良预后相关,可能是AML潜在有效的免疫治疗靶点。

Objective To find potential effective immunotherapy targets for acute myeloid leukemia(AML) by analyzing the expression and clinical significance of different immune checkpoints.Methods Gene expression profiles of AML cell lines(GSE57083) and tissues(GSE37642) were downloaded from GEO database.Then different immune checkpoints expression and clinical significance were analyzed with R studio and Graph Pad software.Results In 16 AML cell lines,the expression rank of 11 immune checkpoints genes was LGALS9,PVRL2,PVR,PDCD1,TIM3,CTLA4,PDL1,GITR,LAG3,PDL2,GITRL,and among which LGALS9,PVRL2,GITR had larger variation between different cells.In AML tissues,the expression rank of immune checkpoint was LGALS9,PVRL2,TIM3,PDCD1,CTLA4,LAG3,GITR,PDL1,PVR,GITRL,PDL2.Survival analysis revealed high expression of PVR and LGALS9 was associated with poor prognosis,while the survival time had no significant difference in other genes.Conclusions PVR is highly expressed in AML tumor cells,and LGALS9 is highly expressed in both AML tumor cells and tissues.High expression of PVR and LGALS9 is associated with poor prognosis of AML patients,which may be a potential effective immunotherapy target for AML.