β-连环素蛋白与神经细胞黏附分子在甲状腺癌中的表达及相关性分析

Expression and correlation of β-Catenin and neural cell adhesion molecule in thyroid carcinoma

目的探究β-连环素蛋白(β-ceatenin)与神经细胞黏附分子(neural cell adhesion molecule ,NCAM)在甲状腺癌的表达及两者的相关性。方法选取2012年12月至2017年12月浙江中医药大学附属温州中西医结合医院普外科收治的62例甲状腺癌组织及同期行手术切除的44例甲状腺腺瘤组织,采用免疫组化染色法检测NCAM及β-catenin在甲状腺癌组织和甲状腺腺瘤组织的表达水平,并分析NCAM及β-catenin的表达水平与甲状腺癌患者临床病理特征的关系及两者的相关性。结果NCAM在甲状腺癌组织的阳性表达率(12.90%)明显低于甲状腺腺瘤组织(90.91%),差异具有统计学意义(t=63.203 ,P=0.000);β-catenin蛋白在甲状腺癌组织的阳性表达率(82.26%)明显高于甲状腺腺瘤组织(6.82%),差异具有统计学意义(t=58.608,P=0.000);NCAM与β-catenin在甲状腺癌中的表达呈负相关(r=-0.220, P=0.024);性别,年龄、肿瘤直径、组织学类型、病理分期不同的甲状腺癌患者,癌组织中NCAM表达水平差别不显著,差异无统计学意义(t=1.960、0.054 3.335、0.807、0.218,P=0.162、0.816、0.069、0.848、0.641);淋巴结转移情况不同的甲状腺癌患者,癌组织中NCAM表达水平差别显著,差异具有统计学意义(t=8.373,P=0.004);性别、组织学类型、肿瘤直径、年龄、淋巴结转移情况、病理分期不同的甲状腺癌患者,癌组织中B-catenin表达水平差别不显著,差异均无;统计学意义(t=0.258、2.307、0.424、0.741、2.570、0.126,P=0.612、0.511、0.515、0.389、0.109、0.722)。结论NCAM在甲状腺癌中呈低表达,β-catenin在甲状腺癌中呈高表达,NCAM与β-catenin在甲状腺癌中的表达呈负相关,且NCAM的表达与甲状腺癌患者淋巴结转移情况呈相关性。

Objective To investigate the expression level and correlation of 0-catenin and neural cell adhesion molecule (NCAM) in thyroid carcinoma. Methods In this study, the expression levels of NCAM and β-catenin in thyroid carcinoma tissues (n=62) and thyroid adenoma tissues (zi=44) collected from patients treated in Wenzhou Integrated Traditional Chinese and Western Medicine Hospital Affiliated to Zhejiang Chinese Medical University from Dec. 2012 to Dec. 2017 were detected by immunohistochemical staining, then its correlation with clinicopathological features was analyzed. Results The positive expression rate of NCAM in thyroid carcinoma tissues was significantly lower than that in thyroid adenoma tissues (12.90% vs 90.91%,t=63.203, P= 0.000). The positive expression rate of β-catenin protein in thyroid carcinoma tissues was significantly higher than that in thyroid adenoma tissues (82.26% vs 6.82%,(=58.608,P=0.000). NCAM and β-catenin were negatively correlated in thyroid carcinoma tissues (r=-0.220, P=0.024). The difference of NCAM expression level was not significant among thyroid carcinoma patients with different gender, age, tumor diameter, histological type or pathological stage(t=1.960, 0.054, 3.335, 0.807, 0.218;P=0.162, 0.816, 0.069, 0.848, 0.641). The expression of NCAM in cancer tissues was significantly different in patients with different lymph node metastasis(t=8.373, P= 0.004). The expression of β-catenin in cancer tissues was not significant in thyroid carcinoma patients with different gender, histological type, tumor diameter, age, lymph node metastasis or pathological stage (t=0.258, 2.307, 0.424, 0.741, 2.570, 0.126;P=0.612, 0.511, 0.515, 0.389, 0.109, 0.722). Conclusions In thyroid carcinoma patients, NCAM is down-regulated, and β-catenin is highly expressed. Moreover, the two indicators are negatively correlated. Additionally, NCAM expression is correlated with lymph node metastasis in thyroid carcinoma patients.