西瓜愈伤组织诱导研究

Study on Callus Induction of Watermelon

为促进西瓜离体快繁和工厂化育苗,以西瓜无菌苗为试验材料,研究影响西瓜愈伤组织形成的条件因素。结果表明:在25℃暗培养条件下,西瓜子叶节最适合进行愈伤组织诱导培养,西瓜子叶节外植体愈伤组织诱导率最高,为87.55%。7d龄期西瓜无菌苗子叶节诱导出的愈伤组织,诱导率最高,达到89.62%,状态良好。西瓜子叶节诱导愈伤组织适宜激素浓度配比为2.0mg·L^-1 2,4-D+1.0mg·L^-1 6-BA。因此,西瓜愈伤组织形成的最佳条件为选用7d龄期西瓜无菌苗子叶节为外植体,在MS+2.0mg·L^-1 2,4-D+1.0mg·L^-16-BA的培养基上进行诱导。

In order to promote rapid propagation in vitro and industrialized seedling raising of watermelon , the MS medium was chosen as the basic culture medium , the cotyledon , cotyledon node and hypocotyl were used as explants , factors affecting callus formation in watermelon were studied.The results showed that under the dark culture condition of 25℃, the cotyledon node of watermelon was most suitable for callus induction.The callus induction rate of cotyledon node was 87.55%.7dwas the most suitable seedling age for callus induction , which had the highest induction rate of 89.62%, and the state of callus was good.The appropriate hormone concen-tration ratio for watermelon callus induction was 2.0mg · L^-1 2 , 4-D+1.0mg · L^-1 6-BA.Therefore , the optimal conditions for the formation of callus are as follows : cotyledon nodes of 7d-age watermelon seedling were used as explants , and cultured in MS basic medium with 2.0mg · L^-1 2 , 4-D+1.0mg · L^-1 6-BA.