茶园间作灵芝对土壤细菌多样性和群落结构的影响

Microbial Diversity and Community Structure in Soil under Tea Bushes-Ganoderma lucidum Intercropping

【目的】探讨茶园间作灵芝对土壤细菌多样性和群落结构的影响,为茶菌间作模式的应用提供科学依据。【方法】应用高通量测序技术分析比较了间作灵芝茶园与单作茶园之间土壤细菌群落变化。【结果】与单作茶园(CK)相比,间作灵芝茶园土壤的有机质、全氮、速效氮和有效磷含量显著提高,速效钾含量和pH显著降低,而全磷和全钾含量变化不显著。间作灵芝茶园处理的土壤细菌群落丰度指数和多样性指数与单作茶园相比无显著性差异。与单作茶园相比,间作灵芝茶园土壤的变形菌门相对丰度显著提高21.18%,而酸杆菌门和芽单胞菌门的相对丰度显著降低15.09%和53.52%,其他细菌门类变化不显著。在属水平上,间作灵芝处理显著提高了土壤有益微生物菌群伯克氏菌属Burkholderia、鞘氨醇单胞菌属Sphingomonas和戴氏菌属Dyella的相对丰度。相关性分析表明,土壤pH、全氮、全磷和速效钾对优势细菌群落的影响较大。【结论】茶园间作灵芝改变了土壤细菌群落结构并提高了土壤中有益微生物菌群的相对丰度,但对土壤细菌群落多样性的影响不明显。

【Objective】Effects of intercropping Ganoderma lucidum among tea bushes on the microbial diversity and community structure in the soil were investigated.【Method】The microbial communities in the monoculture (CK) and tea bush- G.lucidum intercropping soils were compared using the high-throughput sequencing technology.【Result】Compared with CK,the intercropping significantly increased the organic matters,total nitrogen,alkali-hydrolyzed nitrogen,and available phosphorus and significantly reduced the available potassium and pH with no significant effect on the total phosphorus and total potassium in the soil.No significant differences were observed on the richness and diversity indices between them.Furthermore,the relative abundance of Proteobacteria in the intercropping soil significantly increased by 21.18% over CK,while that of Acidobacteria declined by 15.09% and that of Gemmatimonadetes by 53.52%,as none found on those of other bacterial phyla.On genus level,the intercropping significantly increased the relative abundance of beneficial microorganisms,such as,Burkholderia,Sphingomonas and Dyella,in the soil.A correlation analysis showed that the pH,total nitrogen,total phosphorus,and available potassium in soil exerted a greater effect on the dominant than the minor bacterial communities.【Conclusion】 By intercropping G.lucidum among tea bushes,the structure of microbial community in the soil was altered with an increased abundance of beneficial microbial species without significant changes on diversity.