CM-DiI标记的BMSCs在实验性自身免疫性脑脊髓炎大鼠的体内示踪分布

Tracing of CM-DiI labeled BMSCs in experimental autoimmune encephalomyelitis rats

目的观察并探讨CM-DiI标记骨髓间充质干细胞(BMSCs)移植到实验性自身免疫性脑脊髓炎(EAE)大鼠体内的示踪分布。方法全骨髓培养法分离培养大鼠BMSCs;用CM-DiI荧光染料进行体外标记;将标记后的BMSCs移植到EAE大鼠体内,观察移植后细胞的形态及分布。结果荧光显微镜下可在移植BMSCs的EAE大鼠大脑、小脑和脊髓切片内检测到发出红色荧光的细胞,疾病高峰期数量较多,主要位于软膜下和血管周围,形状为圆形或椭圆形;疾病缓解期大部分细胞仍存在,荧光强度略有减弱。结论CM-DiI是一种短中期标记、示踪BMSCs的良好染料,CM-DiI标记的BMSCs在EAE大鼠体内主要分布在血管周围,少量向脑实质内迁移。

Objective To investigate whether CM-DiI labeled BMSCs can be detected in central nervous system(CNS)of MS rat models and how do they migrated.Methods BMSCs were obtained and subsequently co-incubated with whole bone marrow cell culture method.CM-DiI solution was used to label BMSCs in vitro and tagged cells were transplanted into EAE rats by intravenous injection through caudal vein.Tissue sections were prepared and were examined under fluorescence microscope.Results CM-DiI labeled BMSCs mostly distributed around perivascular and subpial areas of spinal cord,cerebrum and cerebellum,showing round or oval shape during peak period.Cells failed to migrate into parenchyma in EAE rats.The duration was as long as 2 weeks after implantation cells can be detected clearly.Conclusions CM-DiI is a good choice for BMSCs labeling and tracing.Labeled cells keep their morphology in vivo and mainly locate around vessels and subpial areas,less migrating into parenchyma of CNS.