摘要
目的:探讨PTEN诱导激酶1(PINK1)沉默对肺癌细胞增殖和凋亡的影响及其机制。方法:将人肺癌细胞 H2106分为3组:空白组(不处理)、阴性组(将shRNA-对照质粒转染入肺癌细胞中)、转染组(将shRNA-PINK1质粒转染入肺癌细胞中)。转染48 h后,检测各组细胞中PINK1等蛋白的表达、细胞增殖抑制率、细胞周期、细胞凋亡。结果:在转染12~72 h时,转染组细胞增殖抑制率高于空白组和阴性组,差异有统计学意义( P<0.05),空白组与阴性组差异无统计学意义(P >0.05)。与空白组和阴性组对比,转染组S期细胞数量降低、G2/M期细胞数量增加、细胞凋亡率降低,差异有统计学意义( P <0.05)。转染组活性caspase3和Bax表达水平增加、PINK1和Bcl-2表达降低,差异有统计学意义( P<0.05),空白组与阴性组间差异无统计学意义(P >0.05)。结论: PINK1沉默可抑制肺癌细胞增殖,诱导癌细胞凋亡,可能是肺癌治疗的另一个新靶点。
Objective: To investigate the effect of PTEN induced putative kinase 1 (PINK1) silencing on proliferation and apoptosis of lung cancer cells and its mechanism. Methods: Human lung cancer cells (H2106) were divided into 3 groups:control group (untreated),negative group (the shRNA-control plasmids were transfected into lung cancer cells),transfection group (the shRNA-PINK1 plasmids were transfected into lung cancer cells).Expression of PINK1 protein and so on,cell proliferation inhibition rate,cell cycle and apoptosis were measured in each group at 48h after transfection. Results: At 12-72 h after transfection,the cell proliferation inhibition rate of the transfected group was higher than that of the control group and the negative group ( P <0.05),but there was no significant difference between the control group and the negative group ( P >0.05).Compared with the control group and the negative group,the number of cells in the S phase were decreased,the number of cells in the G2/M phase were increased,the apoptosis rate was decreased in the transfection group,the differences were statistically significant (P <0.05).The expressions of active caspase3 and Bax were increased,the expression of PINK1 and Bcl-2 were decreased ( P <0.05) in the transfection group,the differences were statistically significant ( P <0.05),but there was no significant difference between the control group and the negative group ( P >0.05). Conclusion: PINK1 silencing inhibits the proliferation of lung cancer cells,induces apoptosis of cancer cells.PINK1 may be another new target for lung cancer treatment.
作者
申秋菊
赵静
王二雄
白晓瑞
SHEN Qiu-ju;ZHAO Jing;WANG Er-xiong;BAI Xiao-rui(Department of Respiratory Medicine,the First Hospital of Yulin,Yulin 719000,Shaanxi,China)
出处
《川北医学院学报》
CAS
2019年第4期351-353,共3页
Journal of North Sichuan Medical College