慢性乙型肝炎患者体内不同来源标本中miR-146a和miR-155的表达

Expressions of miR-146a and miR-155 in different samples of chronic hepatitis B patients

目的:通过检测慢性乙型肝炎(chronic hepatitis B,CHB)患者不同来源标本中miR-146a和miR-155的表达,了解不同来源标本中2种miRNAs表达的相关性,为研究miRNA在肝病中的作用机制提供标本选择的理论依据。方法:采用real-timePCR对41例CHB患者核苷(酸)类似物抗病毒治疗基线期和第104周血浆、外周血单个核细胞和肝组织中miR-146a和miR-155的表达进行检测,分析3种来源标本中这2种miRNAs表达的相关性。结果:治疗第104周时血浆、外周血单个核细胞和肝组织miR-146a和miR-155的表达水平均较基线期显著下调(P<0.05)。基线期血浆和肝组织(r=0.560,P=0.007)、外周血单个核细胞和肝组织(r=0.428,P=0.047)miR-146a的表达均相关。基线期血浆和肝组织(r=0.587,P=0.004)、外周血单个核细胞和肝组织(r=0.483,P=0.023)miR-155的表达相关,miR-146a和miR-155在血浆中的表达与外周血单个核细胞中的表达不相关(P>0.05)。结论:与外周血单个核细胞相比,血浆中miR-146a和miR-155的表达能更好地反映和预测肝组织miR-146a和miR-155的表达,血浆可作为研究miR-146a和miR-155在肝病中作用机制的标本来源。

Objective: To detect the levels of miR-146a and miR-155 in different samples from chronic hepatitis B (CHB),reveal whether there is a correlation between the 2 miRNAs in different samples,and to provide a theoretical basis for sample choice of miRNA research in liver. Methods: Real-time PCR was conducted to examine the expression of miR-146a and miR-155 in the plasma,peripheral blood mononuclear cell (PBMC),and liver tissues from 41 CHB patients who underwent nucleoside analogues antiviral therapy for 104 weeks. Correlations between the levels of miR-146a and miR-155 among the 3 samples were analyzed. Results: The expressions of miR-146a and miR-155 in the plasma,PBMC and liver tissues were significantly down-regulated at the 104th week than those at the baseline (all P<0.05). There was a correlation in the expression of miR-146a between plasma and liver tissues (r=0.560,P=0.007), PBMC and liver tissues (r=0.428,P=0.047) at baseline. There was a correlation in the expression of miR-155 between plasma and liver tissue (r=0.587,P=0.004),PBMC and liver tissue (r=0.483, P=0.023) at baseline. The expressions of miR-146a and miR-155 between the plasma and PBMC were not correlated (P>0.05). Conclusion: Compared with PBMC,miR-146a and miR-155 from plasma can better reflect the expression in the liver tissues,suggesting that plasma can be applied in the mechanism research on miR-146a and miR-155 in the liver diseases instead of liver tissues.