高血压肾损害患者血清IL-22水平变化及其临床意义

Changes in serum IL-22 level in patients with hypertensive renal damage and its clinical significance

目的:通过观察原发性高血压及高血压肾损害患者血清白细胞介素-22(IL-22)浓度变化及与相关指标的关系,探讨其在高血压肾损害中的作用及临床意义。方法:收集97例原发性高血压患者,根据24h尿蛋白定量结果分为单纯高血压组(n=45)和高血压肾损害组(n=52),40例健康体检者为对照组。用酶联免疫吸附试验检测各组血清IL-22水平,流式细胞术检测外周血Th22细胞亚群比例,免疫比浊法测定超敏C反应蛋白(hsCRP)的浓度,并进行相关性分析。结果:与对照组相比,单纯高血压组血清IL-22水平升高,外周血Th22细胞比例明显上升(均P<0.01);高血压肾损害组血清IL-22较单纯单纯高血压组升高(P<0.01),外周血Th22细胞亚群比例也高于单纯高血压组(P<0.05)。直线相关分析显示:单纯高血压组血清IL-22与收缩压、舒张压及hsCRP水平呈正相关(r=0.367,P<0.01;r=0.402,P<0.01;r=0.329,P<0.05),与Th22细胞比例呈正相关(r=0.478,P<0.01)。高血压肾损害组血清IL-22水平与24h尿蛋白定量呈正相关(r=0.318,P<0.05)。结论:高血压肾损害患者血清IL-22水平及外周血Th22细胞比例均较单纯高血压患者升高,血清IL-22水平和血压水平与高血压肾损害的程度呈正相关,提示其可能参与高血压肾损害的发生发展。

Objective: To observe the changes in serum interleukin-22 (IL-22) level and its correlation with other clinical indexes in patients with essential hypertension and hypertensive renal damage,and to investigate its eff ects and clinical signifi cance. Methods: A total of 97 essential hypertension (EH) patients and 40 healthy control were enrolled in this study.Th e EH patients were divided into a simple hypertension (SH) group (n=45) and a hypertensive renal damage (HRD) group (n=52) according to 24 h urinary protein (24 h-UPRO) level.Basic clinical data were collected;serum IL-22 level was detected by enzyme-linked immunosorbent assay (ELISA);the proportion of Th22 cells in peripheral blood was evaluated by flow cytometry (FCM);and serum high sensitivity C-reactive protein (hs-CRP) concentration was measured by immune turbidimetry.Correlation analysis was applied between serum IL-22 level and other indexes. Results: Serum IL-22 level and Th22 cell proportion in patients with hypertension were significantly higher than those in the healthy controls (all P<0.01).Compared with the SH group, serum IL-22 level and Th22 cell proportion were significantly elevated in the HRD group (P<0.01, P<0.05,respectively).Bivariate linear correlation analysis revealed that serum IL-22 level was positively correlated with systolic blood pressure (SBP),diastolic blood pressure (DBP),and hsCRP (r=0.367,P<0.01;r=0.402,P<0.01;r=0.329,P<0.05);there was also a positive correlation between serum IL-22 concentration and Th22 cell proportion (r=0.478,P<0.01) in the SH group. Serum IL-22 level was positively correlated with 24 h-UPRO level (r=0.318,P<0.05) in the HRD group. Conclusion: Serum IL-22 level and peripheral blood Th22 cells are significantly increased in the HRD group compared with the SH group.Serum IL-22 level is closely correlated with extent of hypertensive renal damage,indicating that serum IL-22 level may involve in the pathogenesis of hypertensive renal damage.