麻山药不同生长时期根际土壤微生物多样性及群落结构特征

Microbial Diversity and Community Structure Characteristics of Yam Rhizosphere Soil at Different Development Periods

旨在研究麻山药不同生长时期根际土壤微生物动态变化规律。采用高通量测序的方法,研究了麻山药在苗期、花期和收获期根际土壤微生物群落结构的变化。结果表明细菌多样性和丰富度在不同生长时期内没有显著变化,而真菌变化趋势更为明显,从开始生长期到收获期逐渐增多,在收获期时多样性指数和丰度达到最高。变形菌门和酸杆菌门是麻山药根际细菌群落的最优菌群,两者在不同时期所占比例均超过了 50%,放射毛霉属和被孢霉属是真菌群落的最优菌群,两者在不同时期所占的比例约 15%,结合其他优势菌群在不同生长时期的明显变化,可以看出,这些菌群跟麻山药生长发育密切相关。

This work is to study the dynamic change law of microbial diversity of soil samples at different reproductive periods from yam rhizosphere. By adopting the method of high-throughput sequencing technology,we studied the soil microbial community structure changes of yam root zone in seedling,flowering and harvest stage. Results showed that the diversity and abundance of bacteria in different growth period did not have significant variation,while there was more apparent variation for fungi,and the variation increased gradually from the start growing season to harvest,reached the highest degree of diversity index and abundance in harvest stage. Proteobacteria and Acidobacteria were the dominant bacteria at phylum level,which had over 50% proportion in different periods. Actinomucor and Mortierella were the dominant fungi at genus level,which accounted for 15% proportion in different periods. Combined with the obvious changes of other advantage bacterial group in different growth stages,it can be seen that these flora are closely related to the growth and development of yam.