一株高效好氧反硝化细菌的分离鉴定及脱氮性能研究

Isolation and Identification of an Efficient Aerobic Denitrifying Bacterium

从广州市西朗珠江水产研究所养殖池中分离新型好氧反硝化细菌,旨为开发新型生物脱氮工艺奠定基础。利用 3种不同筛选培养基分离好氧反硝化细菌,测定在单一氮源培养基中发酵的生长曲线和脱氮性能,通过形态和 16S rRNA 基因序列分析对菌株进行鉴定并构建系统发育树。分离得到一株高性能好氧反硝化菌株 BB-17,该菌株在单一氮源培养基中以 28℃,180 r/min 振荡培养时,其对数期出现在 6-24 h,氨氮去除率在 18 h 达 93.92%,硝态氮去除率在 48 h 达 66.11%,亚硝态氮去除率在 48h 达 99.10%。BB-17 为革兰氏阴性短杆菌,16S rDNA 序列分析结果与 Pseudomonas vancouverensis(AJ011507)比对具有 98.41%同源性,确定 BB-17 为假单胞菌属并命名为 Pseudomonas sp. BB17。

New types of aerobic denitrifying bacteria were isolated from the culture pond of the Xilang Pearl River Aquaculture Institute in Guangzhou,aiming at laying the foundation for the development of a new biological nitrogen removal process. Three different screening media were used to isolate the aerobic denitrifying bacteria,and the growth curve and denitrification performance of fermentation in a sole nitrogen source medium were determined. Then morphology and 16S rRNA gene sequence analysis were employed to identify the strains and a phylogenetic tree was constructed. As result,a high-performance aerobic denitrifying strain BB-17 was isolated. When the strain was cultured at 28℃,180 r/min in a sole nitrogen source medium,the logarithmic phase appeared at 6-24 h and the removal rate of ammonia nitrogen was up to 93.92% at 18 h,that of nitrate nitrogen reached 66.11% at 48 h,and that of nitrite nitrogen reached 99.10% at 48 h. BB-17 was a Gram- negative Brevibacterium, the results of 16S rDNA sequence analysis presented 98.41% homology with Pseudomonas vancouverensi( s AJ011507), and BB-17 was identified as Pseudomonas and named P. sp. BB17.