细胞复制性衰老与应激诱导的MRC-5细胞早衰过程的对比研究

A comparative study on the process of cell replication senescence and stress-induced premature senescence of mrc-5 cells

目的:评价比较细胞复制性衰老和应激诱导的MRC-5细胞早衰过程。方法:将MRC-5人胚肺成纤维细胞分为青年对照组(24PDL)、复制性衰老组(50PDL)和H2O2诱导的早衰细胞组,对细胞形态学变化和衰老相关β-半乳糖苷酶染色(SA-β-gal)衰老指标进行综合评价。结果:对于细胞复制性衰老与MRC-5细胞早老模型,二者在衰老过程中所表现出细胞形态学及SA-β-gal染色阳性细胞率等方面的变化是一致的,且应激诱导的MRC-5细胞早衰过程中所表现出的衰老相关变化更为显著。H2O2作为体外诱导剂成功构建MRC-5细胞早老模型(通过相关细胞形态学变化及SA-β-gal染色验证),两种细胞衰老方式在其转录调控机制上有其共同之处。结论:尽管衰老机制不同,但细胞复制性衰老和应激诱导的细胞早衰过程转录调控机制相似,可能与DNA损伤加重或氧化应激水平升高有关。

Objective: To evaluate and compare the process of cellular replicative senescence and stress-induced premature senescence of mrc-5 cells.Methods: mrc-5 human embryonic lung fibroblasts were divided into youth control group (24 PDL), replicative senescence group (50 PDL) and H 2O 2-induced progeria group.Morphological changes and senescence related beta galactosidase staining (SA-beta-gal) were evaluated.Results: The cell morphology and sa-bet-gal positive cell rate in the mrc-5 premature senescence model were consistent with those in the mrc-5 premature senescence model.Senescence related changes in stress-induced premature senescence of mrc-5 cells were more significant.H 2O 2 can be used as an in vitro inducer to successfully construct the mrc-5 cell premature senescence model (verified by relevant cell morphological changes and sa-bet-gal staining). The two cell senescence methods have similarities in transcriptional regulation mechanism.Conclusion: Although the mechanism of senescence is different, the transcriptional regulation mechanism of cell replicative senescence and stress-induced premature senescence is similar, which may be related to the aggravation of DNA damage or the increase of oxidative stress level.