循环microRNA-193簇对乳腺癌的筛查价值

Circulation miR-193 cluster serves as a promising biomarker for diagnosis of breast cancer

目的 探讨循环microRNA-193(miR-193)家族对乳腺癌(BC)的筛查价值。方法 检索GEO数据库并分析miR-193簇的表达。收集45例健康人对照及130例乳腺癌患者血清样本,RT-qPCR检测各组血清miR-193簇的表达水平。ROC曲线分析miR-193簇对BC的筛查效能。结果 在筛选阶段,GEO数据集分析结果表明,BC患者血清中miR-193a-5p与miR-193b表达降低且具有良好的诊断效能;RT-qPCR结果显示,与对照组相比,BC患者血清中miR-193簇成员(miR-193a-3p,-5p和 miR-193b-5p )的含量均显著降低( P 均<0.01)。在验证阶段,miR-193a-5p和miR-193b-5p的AUC ROC 分别为0.888(95% CI : 0.808 ~0.969)和0.954(95% CI :0.902~1.000),而二者联合筛查的AUC ROC 为0.982(95% CI :0.966~0.999)。结论 血清 miR-193a-5p 和miR-193b-5p可以作为BC的筛查标志物,二者联合显著提高筛查效能,具有较好的临床应用潜能。

Objective To evaluate circulating miR-193 cluster as a biomarker for diagnosis of breast cancer (BC). Methods A total of 130 BC patients and 45 healthy controls (HCs) were enrolled. The level of miR-193 cluster was analyzed by using GEO database. RT-qPCR was used to evaluate the level of miR-193 cluster in serum of participants. Receivers operating characteristic (ROC) curve analysis was manipulated to investigate their diagnostic value for BC. Results In the screening stage, the results of GEO database indicated the expression of miR-193a-5p and miR-193b were decreased in the serum of BC patients with prior diagnostic value, and the significant decreased expressions of miR-193 cluster members, miR-193a-3p,-5p and miR-193b-3p, were observed in serum of BC patients compared with those in healthy controls by the determination of RT-qPCR(all P <0.01). In the validation stage, the AUC ROC of miR-193a-5p and miR-193b-5p were 0.888 (95% CI :0.808-0.969) and 0.954 (95% CI :0.902-1.000), respectively. In addition, the combined AUC ROC of these two miRNAs was 0.982 (95% CI :0.966-0.999). Conclusion miR-193a-5p and miR-193b-5p in serum could be served as diagnostic biomarker for screening of breast cancer, and the combined detections of the two miRNAs may exhibit more diagnostic efficiency and good clinical application potential.