葡萄风信子MaHY5基因的克隆与表达分析

Cloning and Expression Analysis of MaHY5 Transcription Factor from Grape Hyacinth

HY5(ELONGATED HYPOCOTYL5)是促进植物光形态建成过程中非常重要的转录因子。该研究以亚美尼亚葡萄风信子( Muscari armeniacum )为材料,克隆出1个bZIP型转录因子基因 MaHY 5(GenBank 登录号MK281355)。生物信息学分析显示, MaHY 5基因开放阅读框为438 bp,编码145个氨基酸,蛋白质分子量约为16.1 kD,理论等电点为9.78,含有一个保守的bZIP特征结构域;MaHY5蛋白属于亲水性蛋白,无信号肽结构,属于非分泌蛋白,可能定位于细胞核。序列比对和进化树分析表明,HY5在进化过程中高度保守。实时荧光定量PCR和高效液相色谱检测分析表明, MaHY 5基因的表达与花青素积累模式基本协同,都在花中优势表达,且在完全开放的花朵中表达较高。推测 MaHY 5基因可能参与了葡萄风信子花色素积累过程。

HY5(ELONGATED HYPOCOTYL5) is an important transcription factor in photomorphogenesis process. We have cloned a bZIP transcription factor gene named MaHY 5 from grape hyacinth( Muscari armeniacum ) and its GenBank accession number is MK281355. Bioinformatics analysis of MaHY 5 gene showed that it contains a 438 bp ORF that encodes a protein of 145 amino acid with an estimated molecular weight of 16.1 kD and an isoelectric point of 9.78. MaHY5 protein was not predicted to have an N-terminal signal peptide or a transmembrane domain and it was predicated to be a hydrophilic protein that is localized to the nucleus. The protein contains an bZIP domain which is highly conserved in evolution process, analyzed by sequence alignment and phylogenetic analysis. The qRT-PCR and HPLC result indicated that the expression of MaHY 5 was general correlated with the mode of anthocyanin accumulation. They were both highly expressed in petals, especially in fully open flowers. Thus, MaHY 5 may play a role in the process of anthocyanins accumulation in grape hyacinth.