帕瑞昔布钠对乳腺癌患者外周血CD4^+CD25^+调节性T 细胞的影响

The Effects of Parecoxib Sodium on Peripheral Blood CD4^+ CD25^+ Regulatory T Cells in Patients with Breast Cancer

目的:探讨COX-2抑制剂帕瑞昔布钠对乳腺癌患者外周血CD4^+CD25^+调节性T细胞的影响。方法:40例Ⅱ期乳腺癌患者均分为观察组和对照组,于手术切皮前15min,观察组给予帕瑞昔布钠40mg、对照组给予等量生理盐水;于麻醉诱导前30min(T1)、术毕(T2)及术后48h(T3)抽取患者外周血,采用流式细胞术检测CD4^+CD25^+Treg细胞的含量,采用ELISA检测血清转化生长因子β(TGFβ)及白细胞介素-10(IL-10)水平。结果:对照组T2、T3时点的CD4^+CD25^+Treg细胞比例、血清TGFβ及IL-10水平均较T1时点显著升高(P<0.01),观察组T2、T3时点的CD4^+CD25^+Treg细胞比例、血清TGFβ及IL-10水平均较同时点对照组显著降低(P<0.01)。结论:帕瑞昔布钠可抑制手术诱导的乳腺癌患者外周血CD4^+CD25^+Treg细胞比例及血清TGFβ、IL-10水平的上调。

Objective: COX-2 inhibitor parecoxib sodium can inhibit the metastasis of breast cancer by inhibiting the production of CD4^+CD25^+ regulatory T cells (Treg).This study intends to investigate the effects of COX-2 inhibitor parecoxib sodium on CD4 +CD25 +Treg cells in patients with breast cancer. Methods: Forty patients with stageⅡbreast cancer were randomly divided into the parecoxib sodium group and the control group.The observation group was given 40mg of parecoxib sodium and the control group was given equal the same volume of normal saline 15 min before skin incision.Peripheral blood of patients was collected 30 min before induction of anesthesia (T1),at the end of operation (T2),and 48h after operation (T3).The amount of CD4 + CD25 +Treg cells was detected by flow cytometry,and serum TGFβ and IL-10 levels were detected by ELISA. Results: At the end of operation (T2) and 48h after operation (T3),the proportion of CD4 ^+CD25 ^+Treg cells and serum TGFβ and IL-10 levels in the control group were significantly higher than those before anesthesia induction (T1)( P <0.01).The proportion of CD4^+CD25 ^+Treg cells in the parecoxib sodium group at the end of operation (T2) and 48h after operation (T3) was significantly lower than that in the control group ( P <0.01).At the end of operation (T2) and 48h after operation (T3),the content of TGFβ and IL-10 in the control group was significantly higher than that before anesthesia induction (T1)( P <0.01).Compared with the control group,the content of TGFβ and IL-10 in the parecoxib sodium group was significantly reduced at the end of operation (T2) and 48h after operation (T3)( P <0.01). Conclusion: The surgical process results in a significant increase in CD4 ^+CD25 ^+ regulatory T cells in peripheral blood of patients with breast cancer.Parecoxib sodium inhibits the proportion of CD4 ^+CD25 ^+ regulatory T cells and the levels of TGFβ and IL-10 in patients with breast cancer.