磁性纳米抗菌剂的制备及其性能

Preparation and properties of magnetic nano-antibacterial agent

采用水热法合成超顺磁性Fe3O4纳米微球,随后在Fe3O4纳米微球表面功能化含有卤胺结构的N,N’-亚甲基双丙烯酰胺与聚丙烯酸共聚物,并进一步用次氯酸钠处理得到Fe3O4@poly(MBAA-co-AA)-Cl抗菌剂.该抗菌剂平均粒径200nm,具有很好的分散性及生物相容性,对大肠杆菌和金黄色葡萄球菌均具有抗菌性.当样品浓度为6.0g/L时,其对大肠杆菌的杀菌率和金黄色葡萄球的杀菌率分别为99.32%和98.93%;样品浓度增加为7.5g/L时,两种细菌的失活率均可达到100%.同时该抗菌剂杀菌速度快、性能稳定、可再生,通过10次循环氯化处理后,其氧化态氯含量几乎与原始值相同,细菌的失活率也均能达到98%以上,是一种优良、高效的纳米抗菌剂.

Superparamagnetic Fe3O4 nanospheres were synthesized by the hydrothermal methodology. Then, these Fe3O4 nanoparticles were modified with N, N’-methylenebisacrylamide and polyacrylic acid copolymers, and Fe3O4 @poly(MBAA-co-AA)-Cl antibacterial agent was obtained by further treatment with NaClO4 solution, which diameter was about 200 nm in size. The antimicrobial agent has good dispersion and biocompatibility, and has antibacterial properties against both Escherichia coli ( E.coli ) and Staphylococcus aureus .( S. aureus ). When the concentration of the sample was 6.0 g/L, the bactericidal rate of both bacterias were 99.32% and 98.93%, respectively. When the sample concentration increased to 7.5 g/L, the inactivation rate of both bacteria could reach 100%. At the same time, this antibacterial agent has the advantages of fast bactericidal speed, stable performance and reproducibility. After 10 times of cyclic chlorination one space more, the content of its oxidized chlorine is almost the same as the original value, and the inactivation rate of bacteria can reach more than 98%. It is an excellent and efficient nano-antibacterial agent.