赤芍总苷对局灶性脑梗死大鼠脑组织Notch信号通路的影响

Effects of total peony glucoside on cerebral Notch signaling pathway in focal cerebral infarction rats

目的探索赤芍总苷对局灶性脑梗死大鼠脑组织Notch信号通路的影响。方法制备脑卒中大鼠并分为5组(模型组、赤芍总苷低剂量组、赤芍总苷中剂量组、赤芍总苷高剂量组和尼莫地平组),另设假手术组大鼠为对照组,每组20只。所有组别连续给药20d。检测各组大鼠的体质量、脑含水量及脑梗死大小以及脑组织的NICD、Hes1和Hes5的蛋白和Hes1和Hes5mRNA表达。结果各组大鼠给药前的体质量差异无统计学意义(P>0.05),治疗后,与对照组相比,模型组体质量降低(P<0.05),NICD、Hes1和Hes5蛋白表达增高(P<0.05),Hes1和Hes5mRNA增高(P<0.05);与模型组相比,赤芍总苷各组及尼莫地平组体质量增高(P<0.05),NICD、Hes1和Hes5蛋白表达均有不同程度降低(P<0.05),Hes1和Hes5mRNA均有不同程度降低(P<0.05),且呈剂量依赖性趋势。结论赤芍总苷可通过抑制脑组织Notch信号通路以治疗大鼠局灶性脑梗死。

Objective To investigate the effects of total paeony glucoside on brain Notch signaling pathway in focal cerebral infarction rats.Methods Stroke rats were prepared and divided into 5 groups:model group, low dosetotal peony glucoside group, medium dosetotal peony glucoside group, and high dosetotal paeony glucoside group, with 20 rats in each group.All groups received the medication for 20 days.The body weight, brain water content, cerebral infarction size, brain tissue NICD, Hes1 and Hes5 proteins and Hes1 and Hes5 mRNA expressions were measured in each group.Results There was no significant difference in body weight before administration within these groups ( P >0.05).After treatment, compared with those in the control group, the body weight significantly decreased ( P< 0.05);NICD, Hes1 and Hes5 significantly increased;Hes1 and Hes5mRNA significantly increased in the model group ( P< 0.05).Compared with those in the model group, the body weight significantly increased ( P< 0.05);the NICD, Hes1 and Hes5 significantly decreased;Hes1 and Hes5mRNA significantly decreased ( P< 0.05) in a dose-dependent manner.Conclusion Total peony glucoside can treat focal cerebral infarction by inhibiting Notch signaling pathway in rat brain tissue.