甘肃不同产地黄芪中毛蕊异黄酮葡萄糖苷的含量测定

Detection for content of calycosin glucoside in astragalus from different areas of Gansu province

目的测定甘肃不同产地黄芪中的毛蕊异黄酮葡萄糖苷含量,为黄芪药材质量评价与优质药材培育提供依据。方法 2017年11-12月在甘肃省不同产地采集黄芪样品30份,参照《中国药典》(2015年版)方法,甲醇液提取毛蕊异黄酮葡萄糖苷,采用高效液相色谱法检测。结果毛蕊异黄酮葡萄糖苷加标回收率89.0%~100.5%,精密度RSD0.52%~2.67%,对照品溶液放置24 h内稳定,30份样品中毛蕊异黄酮葡萄糖苷含量为0.021~0.101 g/100 g、平均含量0.053 g/100 g。结论甘肃黄芪中毛蕊异黄酮葡萄糖苷含量较高,不同产地存在差异。

Objective To detect the content of calycosin glucoside in astragalus from different producing areas of Gansu, and provide basis for planning and evaluating high quality of astragalus and their cultivation. Methods There were 30 astragalus samples collected from different areas in Gansu during November to December, 2017. The calycosin glucoside were extracted with methyl alcohol and detected with high performance liquid chromatography (HPLC) according to Chinese Pharmacopoeia (2015). Results The recovery of standard addition of calycosin glucoside was 89.0%-100.5%, with RSD from 0.52% to 2.67%. The reference control was stable within 24 h. The content of calycosin glucoside was from 0.021 to 0.101 g/100 g among 30 samples with average value of 0.053 g/100 g. Conclusions The content of calycosin glucoside in astragalus is high, and different from various producing areas.