鳕鱼多肽对大鼠皮肤损伤修复的作用及相关机制

Beneficial effect of cod-derived polypeptides (CDPs) on wound healing in a rat model of skin incisions

目的探讨鳕鱼多肽对大鼠皮肤损伤的修复作用及相关机制。方法24只SD大鼠,每只大鼠背部近脊柱皮肤造6 mm直径损伤模型。同一只大鼠背部分为空白对照(生理盐水组)、阳性对照(重组人表皮生长因子组)、实验组(鳕鱼多肽组)。术后用数码显微镜观察创面,用硫酸坐标纸记录创面大小。于术后第3、6、9、12天分别急性处死6只大鼠,行HE染色,反转录聚合酶链反应(RT-PCR)检测。结果第6、12天,鳕鱼多肽组创面明显缩小,与生理盐水组比较,差异有统计学意义(P<0.05);与生理盐水组比较,鳕鱼多肽组第3天表皮细胞生长因子(EGF)、角蛋白14(K14)表达高,第6、12天EGF、K10、K14表达均较高,差异有统计学意义(P<0.05 )。结论鳕鱼多肽促进SD大鼠全层皮肤损伤的修复,可能机制为促进角质形成细胞增生,上调K10、K14、EGF基因表达。

Objective To investigate the wound-healing process in a rat model of skin full-thickness incisions and to detect related possible mechanism. Methods Twenty-four female rats were selected and the dorsal skin of rats was used as the experimental area. A cutaneous excision (6 mm diameter) was made on the back of each animal, close to the cervical area. The dorsal skin of every rat was allocated to three groups which were treated with physiological saline, human recombinant epidermal growth factor (rhEGF), and CDPs, respectively. After making a rat model of skin incisions, we observed the wound healing process, took photos of the wounds under a digital microscope, and use sulfuric graph paper to record the size of every wound. At the 3rd, 6th, 9th, 12th day after modeling, 6 rats were killed, and mRNA expression of K10, K14, and EGF was detected in the skin tissues using a RT-PCR technique. Results At the 6th and 12th day after modeling, there were significant differences between the experimental group and the blank control group (P<0.05). The gene expression of EGF, K-14 in the third day and that of EGF, K-10 and K-14 in the 6th and 12th day were up-regulated compared with control group, and there were significant differences between them (P<0.05). Conclusions CDPs have a beneficial effect on the acceleration of skin wound healing, possibly due to increasing keratinocyte proliferation and up-regulating the expression of K10, K14 and EGF genes.