摘要
目的:建立HPLC法同时测定给喜古讷-6中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量。方法:采用高效液相色谱法Welchrom C18色谱柱(4.6mm×300mm,5μm),以甲醇-0.1%磷酸水溶液(68:32)为流动相,流速1mL·min^-1,检测波长254nm,柱温40℃。结果:5种成分在各自范围内均呈良好的线性关系(r≥O.9991);平均加样回收率95.73%~102.91%,RSD1.3%~1.6%。结论:高效液相色谱法灵敏度高,该实验方法简便快捷、重现性好,为完善蒙药给喜古讷-6汤的质量控制提供依据。
Objective:HPLC method was established for simultaneous determination of Geixigune-6,Aloeemodin,Rodin,Emodin,Chrysophanol,Physcion.Method:High performance liquid chromatography was performed by Welchrom C18 column (4.6mm×300mm,5μm)The aqueous solution of methanol 0.1% phosphoric acid (68:32) was used as the mobile phase,The flow rate was 1mL·min^-1.The detction wavelength was 254nm,and the column temperature was 30℃.Result:The five components showed good linear relationship in their respective range (r≥0.9991).The average recovery was 95.73%~102.91% RSD 1.3%~1.6.Conclusion:High performance liquid chromatography has high sensitivity,this experimental method is simple and fast,with good reproducibility,accurate and reliable results,providing a scientific basis for improving the quality control standard of Mongolian medicine for Geixigune-6 decoction.
作者
文花
刘燕
董武
白玉霞
WEN Hua;LIU Yan;DONG Wu;BAI Yu-xia(Master degree candidate of 2017 from Mongolian Medicine and Pharmacy College of Inner Mongolia University for Nationalities,028000;Master degree candidate of 2018 from Mongolian Medicine and Pharmacy College of Inner Mongolia University for Nationalities,028000;College of Animal Science and Technology,Inner Mongolia University for Nationalities,028000)
出处
《中国民族医药杂志》
2019年第8期41-43,共3页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
内蒙古民族大学特色交叉学群科建设项目(MDXK008)