摘要
Sl0658属于NAC转录因子家族.本研究克隆了Sl0658的C-端特异性末端序列,利用农杆菌介导法将特异性片段导入受体番茄中进行遗传转化,获得干涉表达的转基因番茄.对转基因番茄果实成熟相关指标(包括果实乙烯释放量、果实呼吸速率、果实硬度、可溶性固形物含量等)和果实成熟相关基因表达量进行测定.研究结果显示,Sl0658干涉转基因番茄果实呈现黄色、乙烯释放量和呼吸速率显著降低、果实硬度提高、部分成熟基因表达量降低.表明Sl0658在调控番茄果实成熟过程中发挥重要作用.
Sl0658 belongs to NAC transcription factor family.In this study,the specific C-terminal sequence of Sl0658 was cloned,the specific fragment was transferred into tomato by Agrobacterium mediated transformation to obtain the transgenic tomato with interference expression.The ripening correlation index of transgenic tomato(including fruit ethylene release,fruit respiration rate,fruit firmness,soluble solid content,etc.)and gene expression of fruit maturity related were measured.Results showed that Sl0658 interfering transgenic tomato fruits was yellow,ethylene release and respiration rate decreased significantly,fruit firmness increased,and partial mature gene expression decreased.Sl0658 may play an important role in tomato fruit ripening.
作者
靖乐
黄永
宋玉竹
张金阳
韩芹芹
JING Le;HUANG Yong;SONG Yuzhu;ZHANG Jinyang;HAN Qinqin(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Panxian Agricultural Science Institute,Liupanshui,Guizhou 553000,China)
出处
《昆明理工大学学报（自然科学版）》
CAS
北大核心
2019年第4期84-89,共6页
Journal of Kunming University of Science and Technology(Natural Science)
基金
国家自然科学基金项目(31560559)
