8种中药煎液防治小鼠酒精性肝损伤的作用比较

Comparison of the Prevention and Treatment of Alcoholic Liver Injury in Mice from 8 Kinds of Chinese Medicine Decoctions

从已报道具有明确保肝作用的8味中药中筛选出对酒精性肝损伤保护作用较好的中药。采用56度红星二锅头(12m L/kg·bw)灌胃10 d建立酒精性肝损伤模型,测定小鼠醒酒、醉酒时间和血清谷草转氨酶(AST)、谷丙转氨酶(ALT),采用HE染色观察肝组织病理学变化。结果表明:川芎、五味子和甘草组的醉酒时间较给药前分别延迟了75.64%(p<0.05)、73.87%(p<0.05)和153.58%(p<0.001);川芎、五味子、葛根、葛花、枳椇子、甘草和黄芩组的醒酒时间分别缩短了75.04%(p<0.001)、67.95%(p<0.001)、71.39%(p<0.01)、60.84%(p<0.001)、75.26%(p<0.01)、80.48%(p<0.001)、54.83%(p<0.05)。与肝损伤模型组相比,川芎、白芍、葛根、葛花、甘草和黄芩组的ALT水平分别降低了22.78%(p<0.001)、27.84%(p<0.001)、14.31%(p<0.01)、20.68%(p<0.01)、12.14%(p<0.05)、20.81%(p<0.01);白芍和葛根组AST水平分别降低14.71%(p<0.05)和12.69%(p<0.05)。病理结果显示川芎、白芍、葛根、葛花、甘草和黄芩组肝细胞水肿和坏死显著减少,肝细胞病变程度减轻。本研究表明,在所研究的8种中药中,川芎、白芍和葛根对酒精性肝损伤的保护作用较好,另外发现川芎还有解酒防醉作用。

In this article, Chinese medicines herbs with best protective effect on alcoholic liver injury were screened from eight kinds of Chinese medicines. Alcoholic liver injury model was established by intragastric administration of 56° red star Erguotou wine(12 mL/kg·bw) for 10 days. The drunken time and sobering time of mice, serum aspartate aminotransferase(AST) and Glutamic-oxalacetic transaminase(ALT) levels were determined. Meanwhile, HE staining were performed to observe pathological changes in liver tissue. Compared with pre-administration, the drunken times of Chuanxiong rhizoma, Schisandrae chinensis fructus and glycyrrhizae groups were respectively prolonged by 75.64%(p<0.05), 73.87%(p<0.05) and 153.58%(p<0.001). The sobering times of the Chuanxiong rhizoma, Schisandrae chinensis fructus, Puerariae lobatae radix, fios puerariae, Hovenia acerba,glycyrrhizae and Scutellariae radix groups were respectively shortened by 75.04%(p<0.001), 67.95%(p<0.001), 71.39%(p<0.01), 60.84%(p<0.001) and 75.26%(p<0.01), 80.48%(p<0.001),and 54.83%(p<0.05). Compared with the model group, the ALT levels of Chuanxiong rhizoma, Paeoniae radix alba, Puerariae lobatae radix, fios puerariae, glycyrrhizae and Scutellariae radix group were respectively decreased by 22.78%(p<0.001), 27.84%(p<0.001), 14.31%(p<0.01), 20.68%(p<0.01), 12.14%(p<0.05), 20.81%(p<0.01), and AST levels in paeoniae radix alba, and Puerariae lobatae radix were respectively decreased by 14.71%(p<0.05) and 12.69%(p<0.05). The pathological results showed that the hepatocyte edema and necrosis of Chuanxiong rhizoma, paeoniae radix alba, Puerariae lobatae radix, fios puerariae, glycyrrhizae and scutellariae radix were significantly reduced, and the degree of hepatocyte lesions was alleviated. The present study showed that among the eight Chinese medicines, Chuanxiong, radix paeoniae alba and radix puerariae had a better protective effect against alcoholic-induced liver injury. Moreover, Chuanxiong rhizoma also had effect for treating hangover and preventing drunkenness.