摘要
外膜蛋白酶T(Outer membrane protease T,OmpT)是大肠杆菌外膜的一种蛋白水解酶,是Omptin家族中第一个被解析结构的外膜蛋白,由10条反向平行的β折叠构成中空的桶状结构。OmpT能够识别两个连续的碱性氨基酸,并水解鱼精蛋白和血纤维蛋白溶酶原等。脂多糖(Lipopolysaccharide,LPS)对OmpT蛋白酶功能的激活具有重要作用。研究发现,OmpT对细菌的定植和黏附至关重要。文章围绕OmpT的结构、功能、毒力等方面综述该蛋白当前的研究进展。
Outer membrane protease T(OmpT)is a proteolytic enzyme of Escherichia coli(E.coli)outer membrane,which is the first outer membrane protein of Omptin family to be resolved.Consisting of 10 antiparallelβ-sheets and a hollow barrel,OmpT can recognize two consecutive basic amino acids,hydrolyze protamine and plasminogen.LPS(Lipopolysaccharide)plays an important role in the activation of OmpT protease.Studies had found that OmpT was vital during bacterial colonization and adhesion to hosts.This article focused on the current research progress of OmpT,including corresponding structure,function,and virulence.
作者
王艳
刘娟华
王航
薛菲
高清清
高崧
刘秀梵
WANG Yan;LIU Juanhua;WANG Hang;XUE Fei;GAO Qingqing;GAO Song;Liu Xiufan(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009;Key Laboratory of Avian Bioproducts Development,Ministry of Agriculture and Rural Affairs,Yangzhou,Jiangsu 225009)
出处
《中国家禽》
北大核心
2019年第15期44-51,共8页
China Poultry
基金
国家自然科学基金项目(31672553、31272559)
国家重点研发计划(2017YFD0500203-2、2017YFD0500705)
江苏高校优势学科建设工程资助项目
关键词
外膜蛋白
外膜蛋白酶T
结构
酶学特征
生物学功能
outer membrane protein
outer membrane protease T
structure
enzymatic characteristics
biologicalfunctions