Akt通路在滋养细胞增殖中的调控机制分析

Analysis of the Regulatory Mechanism of Akt Pathway in Trophoblast Cell Proliferation

目的:分析Akt通路在滋养细胞增殖中的调控机制。方法:体外培养滋养细胞,应用MMT检测不同浓度表皮生长因子处理滋养细胞增殖的情况。流式细胞技术检测不同浓度表皮生长因子处理滋养细胞的凋亡情况。使用Akt通路抑制剂LY294002处理后,检测不同组别的滋养细胞增殖情况。结果:不使用LY294002,干预组细胞增殖高于对照组,干预组2细胞增殖高于干预组1;使用LY294002,细胞增殖被抑制,干预组细胞增殖高于对照组,干预组2细胞增殖高于干预组1(P<0.05)。不使用LY294002,干预组细胞凋亡低于对照组,干预组2细胞凋亡低于干预组1;使用LY294002,细胞凋亡得以促进,干预组细胞凋亡均小于对照组,干预组2细胞凋亡低于干预组1(P<0.05)。结论:表皮生长因子可激活Akt通路促进滋养细胞的增殖,降低滋养细胞的凋亡。Akt通路抑制剂能阻断表皮生长因子促进滋养细胞的增殖。

Objective:To analyze the regulatory mechanism of Akt pathway in trophoblast cell proliferation.Method:Trophoblasts cells were cultured in vitro.MTT was used to detect the proliferation of trophoblast cells treated with epidermal growth factor at different concentrations.Flow cytometry was used to detect the apoptosis of trophoblast cells treated with different concentrations of epidermal growth factor.Proliferation of trophoblast cells in different groups was detected after treatment with Akt pathway inhibitor LY294002.Result:Without LY294002,the cell proliferation of the intervention group was higher than that of the control group,and the cell proliferation of the intervention group 2 was higher than that of the intervention group 1.With LY294002,cell proliferation was inhibited.The cell proliferation of the intervention group was higher than that of the control group,and the cell proliferation of the intervention group 2 was higher than that of the intervention group 1.Without LY294002,the apoptosis of the intervention group was lower than that of the control group,and that of the intervention group 2 was lower than that of the intervention group 1.With LY294002,apoptosis was promoted.The apoptosis of the intervention group was lower than that of the control group,and that of the intervention group 2 was lower than that of the intervention group 1(P<0.05).Conclusion:Epidermal growth factor can activate the Akt pathway to promote the proliferation of trophoblast cells and reduce the apoptosis of trophoblast cells.Akt pathway inhibitors can block epidermal growth factor from promoting trophoblast cell proliferation.