长链非编码RNA ZFAS1在非小细胞肺癌中的表达

Expression of Long Chain Non-coding RNA ZFAS1 in Non-small Cell Lung Cancer

目的检测非小细胞肺癌(non-small cell lung cancer. NSCLC)患者癌组织中锌指结构反义转录本1( zine finger antisense 1, ZFAS1)表达水平,探讨ZFAS1 在NSCLC 进展中的生物学作用。旨在探究长链非编码RNA(long non-coding RNA,lncRNA)在非小细胞肺癌组织中的表达水平。方法通过RNA 提取及聚合酶链定量反应,质粒构建及细胞转染,细胞生长曲线和克隆形成和Western blot 检测RACI 蛋白表达等程序,研究60 例非小细胞肺癌组织及癌旁肺组织中ZFASl 的表达水平。结果锌指结构反义转录本1( zine finger antisense 1, ZFAS1)基因敲减后,A549 细胞增殖能力明显减弱(P < 0.01);A549 细胞周期G 期比例升高,S 期比例下降(P < 0.01);A549 细胞凋亡比例明显增加(P < 0.01);A549 细胞迁移和侵袭能力明显下降(P 均< 0.01);A549 细胞中CyclinDI、Bcl2、N-cadherin、ZEBI、Slug 和Twist 基因表达水平均降低(P均< 0.05)。结论 ZFAS1 在NSCLC 患者癌组织中呈高表达。ZFAS1基因敲减诱导细胞周期阻滞、凋亡和抑制上皮-间质转变(epithelialmesenchymaltransition, EMT),减弱NSCLC 细胞增殖、迁移和侵袭能力。与癌旁肺组织相比较之下,ZFASl 在非小细胞肺癌组织中的表达出现了明显的下调。且其表达水平与60 位患者的病例有显著的相关性,包括患者肿瘤的大小、分化程度、淋巴转移与远端转移等。

Objective To detect the expression of zinc finger antisense 1 (ZFAS1) in cancer tissues of patients with non-small cell lung cancer (NSCLC) and to explore the biological role of ZFAS1 in the progression of NSCLC. The aim was to investigate the expression levels of long noncoding RNA (lncRNA) in non-small cell lung cancer tissues. Methods The expression of ZFASl in 60 cases of non-small cell lung cancer (NSCLC) and paracancerous lung tissues was studied by RNA extraction, polymerase chain reaction (PCR), plasmid construction and cell transfer, cell growth curve and clone formation, and Western blot detection of RACI protein expression in 60 cases of non-small cell lung cancer (NSCLC) and paracancerous lung tissues. Result After ZFAS1 ZFAS1 gene knockdown, the proliferation of A549 cells was significantly decreased (P<0.01). The proportion of G phase in A549 cell cycle increased, the proportion of S phase decreased (P<0.01). The apoptosis rate of A549 cells increased significantly (P<0.01).<0.01);The migration and invasion ability of A549 cells decreased significantly (P<0.01). The expression levels of Cyclin DI, Bcl2, N-cadherin, ZEBI, Slug and Twist genes in A549 cells decreased (P<0.05). Conclusion ZFAS1 is highly expressed in cancer tissues of NSCLC patients. ZFAS1 gene knockdown induces cell cycle arrest, apoptosis and inhibition of epithelialmesenchymal transition (EMT), attenuating NSCLC cell proliferation, migration and invasion. Compared with the paracancerous lung tissue, the expression of ZFAS1 in non-small cell lung cancer tissues was significantly down-regulated. The expression level was significantly correlated with the cases of 60 patients, including the size of the tumor, the degree of differentiation, lymphatic metastasis and distant metastasis.