Hsa-miR-139-3p靶基因预测及生物信息学分析

Target gene prediction and bioinformatic analysis of hsa-miR-139-3p

目的对hsa-miR-139-3p 的靶基因进行预测及相关生物信息学分析,为hsa-miR-139-3p 靶基因的实验验证及其调控机制提供理论基础。方法采用3 种软件预测hsa-miR-139-3p 的靶基因并对结果进行功能注释(GO)和信号通路富集分析(KEGG)。结果 hsa-miR-139-3p 成熟序列在各物种间高度保守。获得的133 个靶基因存在于细胞各个组分,主要有转录调控、基因表达调控和蛋白连接等分子功能,并富集于发育生物学过程,涉及黏着斑信号转导以及基因信息处理等信号通路。结论 hsa-miR-139-3p 通过调控靶基因参与人类生命活动和疾病过程的很多方面,尤其生长发育和癌症过程是值得进一步研究的方向。

Objective To predict the target genes of hsa-miR-139-3p and provide the theoretical basis for its experimental verification and regulation study. Methods Three tools were utilized to predict target genes of hsa-miR- 139-3p, which were analyzed by Gene Ontology and KEGG pathway. Results Hsa-miR-139-3p sequence was highly conserved among different species. Totally 133 target genes were identified, which potentially regulated function such as gene transcription, gene expression and protein connection. Target genes were involved in biological development process. KEGG analysis suggested that those targeted genes mainly focused in cellular signaling including FAK pathway. Conclusions Hsa-miR-139-3p may be involved in many substantial biological and pathological process, especially in organism development and carcinogenesis.