摘要
目的研究血清miR-25水平与胃癌(GC)患者的临床病理特征、诊断和预后之间的关系。方法对184例GC患者,56例胃炎患者和78名健康对照者的血清样本进行实时定量聚合酶链反应(RT-qPCR),探讨miR-25水平与GC临床病理特征(包括诊断和预后)的关系。结果与胃炎患者和健康人员相比,GC患者血清miR-25水平显著上调;以cutoff值0.042为标准,miR-25对GC患者的诊断敏感性为69.4%,特异性为80.4%;高血清miR-25水平与GC浸润深度和淋巴结转移相关,miR-25水平升高是患者预后不良的表现。结论血清miR-25水平是胃癌诊断和判断预后的一项可靠指标。
Objective To study the relationship between serum miR-25 levels and clinicopathological features,diagnosis and prognosis in patients with gastric cancer(GC). Methods Real-time quantitative polymerase chain reaction(RT-qPCR) was performed on serum samples from 184 patients with GC,among them 56 were of gastritis and 78 of healthy controls,and to investigate their relation between miR-25 levels and clinicopathological features (including diagnosis and prognosis). Results Compared with the patients with gastritis and healthy controls,serum miR-25 levels were significantly up-regulated with a cutoff value of 0.042 in the patients with GC. The diagnostic sensitivity of serum miR-25 to GC patients was 69.4%,specificity was 80.4%;high serum miR-25 levels were associated with GC infiltration depth and lymph node metastasis,and elevated serum miR-25 levels could prognose a poor outcome of patients. Conclusion Serum miR-25 level is a reliable indicator for diagnosing and judging prognosis of gastric cancer.
作者
毕书祝
杨越
牛平
王梅
BI Shu-zhu;YANG Yue;NIU Ping(Navy 92426 Troop Hospital,Internal Medcine,Qingdao,Shandong 266400,China)
出处
《实用医药杂志》
2019年第9期779-782,共4页
Practical Journal of Medicine & Pharmacy
关键词
胃癌
预后
诊断
血清miR-25
Gastric cancer
Prognosis
Diagnosis
Clinical pathology
Serum miR-25