龙岩市239株沙门菌毒力基因检测结果分析

Virulence profile in 239 Salmonella isolates in Longyan, Fujian Province

目的了解龙岩市沙门菌毒力基因携带与变迁情况,为沙门菌病的防制提供理论依据。方法 对1991-2017年间收集的分离自人体与食品样本的239株沙门菌进行复核鉴定,并用PCR方法检测 invA、sopB、sifA、sscA、sseE、spvB、spvC、spvR、pefA 等9个沙门菌毒力基因的片段。结果 龙岩市沙门菌以肠炎沙门菌和鼠伤寒沙门菌为主,占62.3%(149/239),属于SPI1的 invA、sopB 毒力基因检出率为100%,属于SPI2的 sseE、sscA、sifA 毒力基因的检出率分别为99.2%、95.0%、85.4%,毒力质粒基因 spvC 检出率71.1%, pefA、spvB、spvR 检出率均为46.4%。并且其携带数量也随着时间的进程而显著增加;肠炎沙门菌质粒毒力基因携带率显著高于鼠伤寒沙门菌。肠炎沙门菌以检出全部9种毒力基因为主,占83.5%(76/91);鼠伤寒沙门菌以 invA、sopB、sseE、sscA、sifA阳性,spvB、spvR、pefA 阴性结果多见,占77.6%(45/58)。人体与食品样本来源的沙门菌所携带毒力基因数量没有差异。结论 龙岩市沙门菌携带毒力基因数量较多,毒力较强,质粒毒力基因随着时间的进程而累积,人源性与食源性沙门菌交叉污染严重,必须加强人、禽、畜沙门菌病的监测与管理。

To understand the carrying and change of virulence genes of Salmonella in Longyan and provide theoretical data for the prevention and control of salmonellosis, 239 strains of Salmonella were isolated from the sample of human body and food from 1991 to 2017 were examined, and the nine fragments of Salmonella virulence gene, invA,sopB, sifA, sscA, sseE, spvB, spvC, spvR, pefA were detected by PCR. Results showed that S. enterica and S. typhimurium were the main Salmonella in Longyan, accounting for 62.3%(149/239). The detection rate of invA, sopB virulence gene of SPI1 was 100%;the detection rates of sseE, sscA and sifA virulence genes of SPI2 were 99.2%, 95.0% and 85.4% respectively;the detection rate of virulence plasmid gene spvC was 71.1% and the detection rates of pefA, spvB and spvR were 46.4%, and the carrier number increased with the progress of time. The carrier rate of virulence gene in plasmid of S. enteritis was significantly higher than that of S. typhimurium . S. enteritidis mainly detected 9 virulence genes, accounting for 83.5%(76/91). The result of which invA,sopB,sseE,sscA,sifA were positive and spvB、spvR、pefA were negative was most common in S. typhimurium , accounting for 77.6%(45/58).There was no difference in the quantity of the virulence genes carried by Salmonella from human and food samples. In conclusions. The number of virulence gene of Salmonella in Longyan is more and the virulence is stronger, and the plasmid virulence gene increases significantly with time going on. The cross-contamination of human and food-borne Salmonella is serious. It is necessary to strengthen the monitoring and management of human, poultry and livestock salmonellosis.