摘要
从开口箭新鲜根茎中分离得到8个化合物,通过理化性质分析、质谱、红外光谱及核磁共振波谱检测等方法鉴定了其结构,分别为开口箭皂苷J(1)、(25S)-26-O-β-D-吡喃葡萄糖基呋甾-1β,3β,22α,26-四羟基-3-O-β-D-吡喃葡萄糖苷(2)、洋地黄毒苷元-3-O-α-L-吡喃岩藻糖苷(3)、弯蕊皂苷元B(4)、异罗斯考皂苷元(5)、罗斯考皂苷元(6)、香豌豆酚(7)及(+)-儿茶素(8).其中,化合物1为新化合物,化合物3,4,7和8为首次从该属植物中分离得到.在抗人结肠癌细胞LoV o和胃癌细胞BGC-823活性评价中,化合物5和6表现出较强的抗肿瘤活性,半抑制浓度(IC50)值分别达到0. 532和0. 757μmol/L.
Tupistra chinensis(Liliaceae), mainly distributed in southwestern China, is a rich source of steroidal sapogenins, saponins and flavans. These components exhibit diverse biological activities such as anti-inflammatory, anti-fungal and anti-tumor properties. As a part of a continuing study for the discovery of novel cytotoxic agents, eight compounds were isolated from the fresh rhizomes of Tupistra chinensis, and their structures were established on the basis of chemical methods and spectral data(MS, IR, NMR), including tupistroside J(1),(25S)-26-O-β-D-glucopyranosyl-furost-1β,3β,22α,26-tetraol-3-O-β-D-glucoside(2), digitoxigenin-3-O-α-L-fucopyranose(3), watti-genin B(4), epiruscogenin(5), epiruscogenin(6), orobol(7),(+)-catechin(8). Compound 1 was a new compound, and compounds 3, 4, 7 and 8 were isolated from Tupistra Ker-Gawl for the first time. All compounds were tested in vitro for their cytotoxic activities against the Human LoVo and BGC-823 cell lines, and compounds 5 and 6 were found to possess significant cytotoxicity against the tested tumor cell lines with half maximal inhibitory concentration(IC50) values of 0.532 and 0.757 μmol/L, respectively.
作者
肖艳华
张广杰
宗良
刘国宏
任丽君
董俊兴
XIAO Yanhua;ZHANG Guangjie;ZONG Liang;LIU Guohong;REN Lijun;DONG Junxing(Institute of NBC Defence,Beijing 102205,China;Academy of Military Medical Sciences,Beijing 100850,China)
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2019年第9期1897-1903,共7页
Chemical Journal of Chinese Universities
基金
国家科技重大专项(批准号:2013ZX09J13101-02B)资助~~
关键词
开口箭
化学成分
抗肿瘤活性
Tupistra chinensis baker
Chemical constituent
Antitumor activity