聚合白背飞虱和褐飞虱抗性基因创制杂交水稻恢复系

Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers

【目的】为了创制兼抗白背飞虱和褐飞虱的水稻恢复系,【方法】分别以抗褐飞虱材料B5(携带褐飞虱抗性基因Bph14和Bph15)及携带白背飞虱抗性位点qsI-4的籼型恢复系福恢7011为供体亲本,以骨干恢复系福恢676为轮回亲本,应用低世代分离群体田间表型结合单株鉴定与高世代稳定株系室内筛选和分子标记辅助选择相结合的方法,并对抗虫株系及其测交后代进行考查和农艺性状分析。【结果】选育出聚合Bph14、Bph15和qsI-4的恢复系材料3份,携带2个抗虫基因的恢复系材料3份。其中6份恢复系的褐飞虱抗性鉴定结果均表现中抗以上。通过抗性鉴定和杂交后代农艺性状分析筛选出具有生产应用潜力的恢复系材料2份。【结论】为褐飞虱和白背飞虱抗性聚合新种质的创制和应用提供了基础材料。

【Objective】To breed restore lines of hybrid rice resistant to white backed planthoppers and brown planthoppers,【Method】we used B5 with brown planthopper resistance genes Bph14 and Bph15 and Fuhui 7011 with white backed planthoppers resistance gene qsI-4 as donor parents, and Fuhui 676, a main restorer line, as the recurrent parent through the molecular marker-assisted selection combined with field and indoor identification. The insect-resistant lines and their hybrid progenies were tested and their agronomic traits were analyzed.【Result】Three restorer lines with three resistance genes to rice brown planthoppers and white backed planthoppers(Bph14, Bph15 and qsI-4) and three lines with two resistance genes were bred. Resistance identification indicated that the resistance of the restorer lines was moderately resistant or resistant. Two restorer lines with potential for production and application were screened out by examining the resistant strains and their crossing progenies and analyzing their agronomic traits.【Conclusion】This study provides new resistance germplasms against brown planthoppers and white backed planthoppers for hybrid rice breeding.