采用聚丙烯酰氨凝胶电泳技术鉴别龟甲及其混伪品

Identification of Tortoise Shells and Counterfeit Products by Polyacrylamide Gel Electrophoresis(SDS-PAGE)

目的:从蛋白水平上对龟甲药材及其混伪品进行鉴别,分析龟甲及其混伪品的差异。方法:本研究采用聚丙烯酰氨凝胶电泳(SDS-PAGE)技术对龟甲及其混伪品进行鉴别,并对龟甲的背甲与腹甲、不同批次正品龟甲药材进行对比分析。结果:对于同一品种的龟甲,其背甲与腹甲的蛋白质亚基相似,电泳得到的蛋白质条带几乎无明显差别;不同批次正品龟甲在一维电泳中显现出的蛋白亚基条带差异性很小,其电泳蛋白条带受产地、批次等因素影响较小;正品龟甲在14~19 kDa,有3条高丰度蛋白聚合在一起,这“二细一粗三聚合”的条带群,可以作为鉴别正品龟甲和其他龟甲混伪品的初步依据。结论:结果显示,SDS-PAGE电泳技术可以对龟甲及其混伪品药材进行初步的鉴别,利用蛋白条带的区别可对其进行区分。

Objective: To identify tortoise shell and its adulterants at the protein level,and analyze the differences between tortoise shell and its adulterants. Methods: In this study,polyacrylamide gel electrophoresis (SDS-PAGE )was used to identify tortoise shells and their mixed products.The tortoise shells and plastrons and different batches of authentic tortoise shell were compared. Results: For the same species of tortoise shell,the protein subunit of dorsal and abdominal shells are similar,and there was almost no significant difference in the protein bands obtained by electrophoresis.The protein subunits of different batches of genuine tortoise shell showed little difference in one-dimensional electrophoresis,and the electrophoretic protein bands were less affected by factors such as origin and batch.There were three high abundance proteins aggregated between 14 and 19 kDa.The band group of " two fine and one rough trimerization" can be used as a preliminary basis for identifying genuine tortoise shells and other adulterated tortoise shells. Conclusion: The results show that SDS-PAGE electrophoresis can be used to identify the tortoiseshell and its mixed counterfeit medicinal materials,and distinguish them by the difference of protein bands.