九香虫及相似品的多糖组成分析

Analysis of Polysaccharide Composition of Aspongopus chinensis and Its Adulterants

目的:分析九香虫及相似品的多糖组成。方法:采用水提醇沉法提取多糖,利用苯酚-硫酸法测定多糖含量,通过柱前衍生化HPLC分析单糖组成,使用SIMCA-P软件进行主成分分析(PCA)。结果:小皱蝽的多糖含量相对偏高,其次为无刺蝽;九香虫等的多糖主要由甘露糖、鼠李糖、葡萄糖、半乳糖等单糖组成,而小皱蝽的多糖主要由葡萄糖组成;从主成分分析来看,除九香虫外,其他品种能聚合为一类。结论:九香虫及相似品多糖含量、单糖组成存在明显差异。

Objective: To analyze the polysaccharide composition of Aspongopus chinensis and its adulterants. Methods : Polysaccharides were extracted by water extraction and alcohol precipitation method.The content of polysaccharides was determined by phenol-sulfuric acid method.The composition of monosaccharides was analyzed by pre-column derivatization HPLC method,and the principal component analysis (PCA )was performed by SIMCA-P software. Results: The polysaccharide content of the Cyclopelta parva was relatively high,followed by Megymenum inerme .The polysaccharides such as A.chinensis were mainly composed of mannose,rhamnose,glucose,galactose and other monosaccharides,while the polysaccharides of C.parva were mainly composed of Glucose composition;from the principal component analysis,in addition to the A.chinensis ,other varieties were aggregated into one class. Conclusion: There are significant differences in polysaccharide content and monosaccharide composition between A.chinensis and its adulterants.