利用ISSR和SSR标记分析不同抽薹性萝卜的遗传多样性

Analysis of Genetic Diversity in Radish (Raphanus sativus L.) with Different Bolting-resistance Based on ISSR and SSR

[目的]本研究加强对耐抽薹萝卜种质资源的研究,加快耐抽薹萝卜育种进程。[方法]筛选出16对ISSR和21对SSR分子标记引物,用于64份不同抽薹性萝卜材料进行遗传多样性分析。[结果]ISSR-PCR共扩增出90条带,其中具有多态性的68条,多态性条带百分率为75.56%;SSR-PCR共扩增出86条带,其中具有多态性的62条,多态性条带百分率为72.09%。POPGENE分析结果表明,两类引物扩增结果都反映出供试材料较高的遗传多样性。UPGMA法聚类结果中,均可把供试萝卜材料分为4个类群,7个亚类。其中,ISSR标记聚类结果与供试萝卜材料的根叶类型比较接近;SSR标记聚类结果与材料的地理分布相似;整合ISSR和SSR后的聚类结果能够反映出2种标记的特征。Mantel检验显示,2种分子标记的遗传相似性系数在64份不同抽薹性萝卜材料间相关不显著(R=0.171,P>0.05),而ISSR+SSR与ISSR标记(R=0.612,P<0.05)以及SSR标记(R=0.894,P<0.05)呈显著相关。[结论]2种标记整合后的聚类分析,可检测到更大的遗传变异;而不同抽薹性萝卜材料所表现出的丰富多样性,对萝卜种质资源管理和耐抽薹品种选育具有重要意义。

[Objective]This study was to strengthen the research on the germplasm resources of bolting-resistant radish and accelerate the breeding of bolting-resistant radishes.[Method]16 pairs of ISSR and 21 pairs of SSR molecular marker primers were screened for genetic diversity analysis of 64 different bolting-resistant radish materials.[Result]A total of 90 bands by ISSR-PCR were amplified,and 68 bands among them were polymorphic,of which the percentage of polymorphic bands was 75.56%.Meanwhile,a total of 86 bands by SSR-PCR were amplified,and 62 bands among them were polymorphic,of which the percentage of polymorphic bands was 72.09%.The amplification results of both primers by POPGENE analysis reflected the higher genetic diversity of materials.UPGMA-based cluster analysis using both ISSR and SSR revealed that 64 materials could be grouped into four main cluster groups and seven inferior groups.The ISSR clustering was more consistent with the root and leaf of materials.The SSR clustering matched similarly with the geographical distribution of materials.And the clustering results after integrating ISSR and SSR could reflect the characteristics of two markers.Mantel's test showed that the correlation coefficient of GS between ISSR and SSR markers were not significantly correlated(R= 0.171,P>0.05).While between ISSR + SSR and ISSR markers(R=0.612,P<0.05),ISSR+ SSR and SSR markers(R=0.894,P<0.05)showed significantly correlated.[Conclusion]The cluster analysis combining both two markers was more suitable for the analysis of genetic diversity on the accessions.The rich diversity of different radish materials was a great significance for the management of radish germplasm resources and the breeding of bolting-resistant varieties.