摘要
[目的]本研究旨在克隆获得藏山羊AQP7基因序列,并构建组织表达谱。[方法]利用RT-PCR技术克隆藏山羊AQP7基因序列,利用相关生物学软件进行生物信息学分析,同时利用qPCR检测AQP7在藏山羊各组织中的表达差异。[结果]克隆获得藏山羊AQP7基因序列长度为1119 bp,其中CDS区为993 bp,编码330个氨基酸。组织表达谱显示AQP7在藏山羊各组织中均有表达,其中在脂肪和肾脏组织中表达量最高,极显著高于其他组织(P<0.01)。[结论]本试验研究结果为藏山羊肌内脂肪研究提供了分子理论基础。
[Objective]The aim of this study was to clone and obtain the AQP7 gene sequence of Tibetan goats,and to construct tissue expression profiles.[Method]The AQP7 gene sequence of Tibetan goats was cloned by RT-PCR and bioinformatics analysis was performed through relevant biological software.Quantitative real-time PCR(qPCR)was used to detect the expression differences of AQP7 in Tibetan goats.[Result]The length of the AQP7 gene sequence was 1119 by,and the length of the CDS region was 993 by,encoding 330 amino acids.The tissue expression profiling showed that AQP7 was expressed in all tissues of Tibetan goats,with the highest expression in Adipose Tissue and kidney Tissues,and significantly higher than other tissues(P<0.01).[Conclusion]The results of this study provide molecular theoretical basis for the study of intramuscular fat in Tibetan goats.
作者
张亚楠
王永
朱江江
许晴
林亚秋
ZHANG Ya-nan;WANG Yong;ZHU Jiang-jiang;XU Qing;LIN Ya-qiu(College of Life Science and Technology,Southwest Minzii University,Sichuan Chengdu 610041 , China;Key Laboratory of Qinghai- Tibetan Plateau Aninul Genetic Resource Reservation and Utilization (Southwest Minzu University), Ministry of Education, Sichuan Chengdu 610041, China)
出处
《西南农业学报》
CSCD
北大核心
2019年第8期1956-1960,共5页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31672395)
四川省科技支撑计划“十三五育种攻关”项目(2016NYZ0045)
中央高校基本科研业务费专项基金项目(2017NGJPY06)