油茶根正丁醇萃取部位的化学成分研究

Study on Chemical Constituents of n-Butanol Part from the Roots of Camellia oleifera

目的:研究油茶根正丁醇萃取部位的化学成分,为阐明其抗肿瘤的药效物质基础提供参考。方法:以95%乙醇为溶剂对油茶根进行提取得到醇提物,依次用乙酸乙酯、水饱和正丁醇溶液萃取醇提物后得到正丁醇萃取部位,然后利用D101大孔树脂柱以及硅胶柱色谱、常压反相柱色谱、葡聚糖凝胶SephadexLH-20柱色谱和制备液相色谱等方法对油茶根正丁醇萃取部位的化学成分进行分离、纯化,并通过理化常数测定以及电喷雾-质谱、核磁共振氢谱、核磁共振碳谱等波谱分析鉴定所得化合物的结构。结果:从油茶根正丁醇萃取部位中分离得到8个化合物,分别鉴定为槲皮素-3′-O-β-D-葡萄糖苷(化合物1)、芹菜素-7-O-β-D-葡萄糖苷(化合物2)、(+)-南烛木树脂酚-3α-O-β-D-葡萄糖苷(化合物3)、甜叶悬钩子苷(化合物4)、杜尔可苷B(化合物5)、4-羟基-3-甲氧基苯酚1-O-β-D-[6-O-(4-羟基-3,5-二甲氧基苯甲酰基)]-吡喃葡萄糖苷(化合物6)、3,4,5-三甲氧基苯基-6-O-紫丁香酰基-β-D-葡萄糖苷(化合物7)和gordonoside P(化合物8)。结论:化合物1~8均为首次从油茶中分离得到。本研究结果不仅丰富了该属植物的化学成分,并且为该部位抗肿瘤药效物质的阐明提供了参考。

OBJECTIVE:To study the chemical constituents of n-Butanol part from the roots of Camellia oleifera,and to provide reference for elucidating the pharmacodynamic substance basis of it’s anti-tumor effect. METHODS:The ethanol extracts were obtained by using 95% ethanol as extraction solvent to extract the roots of C. oleifera,and the n-Butanol part was obatined after the extracts were extratced with ethyl acetate and water-saturated n-butanol solution in turn. The chemical constituents of nbutanol part were isolated and purified by D101 macroporous resin column,silica gel,atmospheric pressure reversed phase column chromatography,sephadex gel SephadexLH-20 column chromatogram and preparative HPLC. The structure of the compounds was identified by spectroscopic analysis of physicochemical constants,electrospray ionization mass spectrometry,1 H-NMR and13 CNMR. RESULTS:Eight compounds were isolated from the roots of C. oleifera and elucidated as quercetin 3′-O-β-D-glucoside(compound 1),apigenin-7-O-β-D-glucoside(compound 2),(+)-lyoniresinol-3α-O-β-D-glucopyranoside(compound 3),rubusoside(compound 4), dulcoside B(compound 5), 4-hydroxy-3-methoxyphenol 1-O-β-D-[6-O-(4-hydroxy-3,5-dimethoxylbenzoate)-glucopyranoside(compound 6),3,4,5-trimethoxyphenyl-6-O-syringoyl-β-D-glucopyranoside(compound7),gordonoside P(compound 8). CONCLUSIONS:Compounds 1-8 were isolated from this plant for the first time. This not only enriches the chemical constituents of this genus,but also provides a reference for elucidating the anti-tumor bioactive substances in this part.