人卵巢癌细胞株3AO原位移植瘤模型的建立

Establishment of orthotopic transplantation tumor model of human ovarian cancer cell line 3AO

目的研究人卵巢癌生长特点和转移范围,构建卵巢癌原位移植瘤动物模型。方法将对数生长期的1×10^7个人卵巢黏液性腺癌3AO细胞种植于BALB/C(nu/nu)雌性裸鼠右卵巢实质内,观察裸鼠一般情况及肿瘤生长、浸润转移情况,移植后3~8周解剖裸鼠,对双侧卵巢和各脏器行光镜和免疫组织化学检查。结果人卵巢黏液性腺癌3AO细胞裸鼠原位移植瘤模型成功率为86.67%。移植后3周可见移植侧卵巢稍增大,逐渐形成团块,并转移至对侧卵巢、肠管、肝脾、肺、淋巴结等器官,有无色透明腹腔积液形成,1~3mL。光镜下观察,移植瘤和转移瘤的病理特征符合人卵巢黏液性腺癌表现,免疫组织化学检测人糖类抗原CA125呈阳性或强阳性表达,着色部位定位于细胞浆。结论人卵巢黏液性腺癌细胞株3AO裸鼠原位移植瘤模型成功建立,该模型与人卵巢癌的生物学行为类似,可以作为进行人卵巢癌研究的体内模型。

Objective In order to study occurrence and matastasis of human ovarian carcinoma,a kind of animal model was establish via orthotopic transplantation of human ovarian mucinous adenocarcinoma 3AO cell lines.Methods Human ovarian mucinous adenocarcinoma 3AO cells(1×10^7)in exponential phase of growth were inoculated in right ovary of BALB/C(nu/nu)female.The general condition,growth and metastasis of tumor were observed.Nude mice were anatomized at 3-8 weeks after transplation,inspection by microscopy and immunohistochemical analysis were applied to both ovaries and organs.Results The success rate of orthotopic transplanted tumor model was 86.67%.Three weeks after transplantation,the right ovary became large,it grew into mass and metastasized to left ovary,intestines,liver,spleen,lung and lymph nodes,1-3 mL ascites appeared colorless.The pathology features of transplated tumors and matastatic tumors were consistence with human ovarian mucinous adenocarcinoma by light microscopy.The expression of human carbohydrate antigen 125 were positive or strong positive.The stain areas were located in cytoplasm.Conclusion The orthotopically transplanted nude model by human ovarian mucinous adenocarcinoma 3AO cells is established successfully.It is similar to human ovarian carcinoma in biological mode,and can be a good in vivo modle in research of human carcinoma.