5-羟甲基糠醛、糠醛、乙酰呋喃、呋喃酮、5-甲基糠醛的高效液相检测方法

High Performance Liquid Phase Method for Determination of Pentahydroxymethylfurfural,Furfural,Acetylfuran,Furanone and Pentamethylfurfural

在高效液相色谱检测呋喃酮方法的基础上,建立多呋喃物质(5-羟甲基糠醛、糠醛、乙酰呋喃、呋喃酮、5-甲基糠醛)的检测方法。色谱柱为UltimateXB-C18柱(250mm×4.6mm,5μm),柱温为35℃,进样量10μL,波长285nm,流速0.8mL/min。脱洗梯度条件:0~2min,甲醇-0.5%甲酸(15∶85,体积比);2min^24min,甲醇-0.5%甲酸(50∶50,体积比);24min^27min,甲醇-0.5%甲酸(100∶0,体积比);27min^29min,甲醇-0.5%甲酸(100∶0,体积比);29min^33min,甲醇-0.5%甲酸(15∶85,体积比)。检测5-羟甲基糠醛、糠醛、呋喃酮、乙酰呋喃、5-甲基糠醛的回收率分别为97.8%~99.6%、95.4%~97.3%、96.3%~97.2%、95.3%~98.3%、94.3%~97.9%,变异系数分别为3.3%、4.3%、0.89%、5.3%、1.4%。适合检测5-羟甲基糠醛、糠醛、呋喃酮、乙酰呋喃、5-甲基糠醛5种呋喃物质。

Based on the high performance liquid phase method for the determination of furanone, a method for the determination of polyfuran (pentahydroxymethylfurfural, furfural, acetylfuran, furanone, pentamethylfu-rfural) was established.The column was an Ultimate XB-C 18 column (250 mm×4.6 mm, 5 μm) with a column temperature of 35 ℃, an injection volume of 10 μL, a wavelength of 285 nm, and a flow rate of 0.8 mL/min.Dewashing gradient conditions: 0-2 min, methanol-0.5 % formic acid (15 ∶ 85,volume ratio);2 min-24 min, methanol -0.5 % formic acid (50 ∶ 50,volume ratio);24 min-27 min,methanol -0.5 % formic acid (100 ∶ 0, volume ratio);27 min-29 min,methanol -0.5 % formic acid(100 ∶ 0,volume ratio);29 min-33 min, methanol -0.5 % formic acid (15 ∶ 85, volume ratio).The recovery rates of pentahydroxymethylfurfural,furfural,furanone,acetylfuran and pentamethylfurfural were measured:97.8 %-99.6 %,95.4 %-97.3 %,96.3 %-97.2 %,95.3 %-98.3 %,94.3 %-97.9 %,The coefficient of variation was 3.3 %,4.3 %,0.89 %,5.3 %,1.4 %.The five furan substances(pentahydroxydroxymethyl-furfural, furfural,acetylfuran, furanone, pentamethylfurfural) were suitable for detection.